sufS
168
cysteine desulfurase, cysteine:[protein|search|SufU ]sulfuryl transferase
locus
BSU_32690
Molecular weight
44.76 kDa
pI
5.2
function
formation of iron-sulfur clusters in proteins
product
cysteine desulfurase
essential
yes
ec
2.8.1.7
synonyms
sufS, yurW, csd
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0520 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,357,444 3,358,664
Phenotypes of a mutant
essential [Pubmed|12682299]
terminal phenotype of a CRISPRi knockdown mutant is moderate bulging [Pubmed|27238023]
the mutant is viable and Fe-S clusters are dispensable if [protein|search|IspG ]and [protein|search|IspH ]from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate [pubmed|29292548]
The protein
Catalyzed reaction/ biological activity
transfer of iron to [protein|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU] [Pubmed|21236255]
[sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)
Protein family
[wiki|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
pyridoxalphosphate [Pubmed|25447671]
Structure
[PDB|5J8Q] [Pubmed|27382962]
[PDB|5XT5] (the [protein|88ACE6B79534338F7C72F107B35A0B2384007088|sufS]2-[protein|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]2 complex) [pubmed|29235855]
[PDB|5ZS9] (resting state)
[PDB|5ZSK] (63 s soak with cysteine)
[PDB|5ZSO] (90 s soak with cysteine)
[PDB|6KFZ]
[AF|O32164]
Effectors of protein activity
interaction with [protein|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU] enhances the activity of [protein|88ACE6B79534338F7C72F107B35A0B2384007088|sufS] 40-fold [Pubmed|20097860]
Paralogous protein(s)
[protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS], [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]
Expression and Regulation
Operons
genes
[gene|E966F3808222820C0644DC98F95765B89E6A2551|sufC]-[gene|1A9DA839AB77AF53958CB8426607BEF14F62B85E|sufD]-[gene|88ACE6B79534338F7C72F107B35A0B2384007088|sufS]-[gene|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]-[gene|5991F051C5BAD61A0860BBC64A37DB9D16130998|sufB]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|22383849], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B585 (yurW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1584 NBRP B. subtilis, Japan]
BEC32690, a CRISPRi knockdown mutant of ''[gene|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]'', is available from the [http://bgsc.org/ Bacillus Genetic Stock Center]
References
Reviews
Original Publications
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Time of last update: 2024-10-04 19:06:24
Author of last update: Jstuelk