pbpH
168
class B penicillin-binding protein H, required for [wiki|cell wall synthesis] during cell elongation, works together with [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA] in the [wiki|elongasome] for lateral [wiki|cell wall synthesis] and control of cell diameter
locus
BSU_13980
Molecular weight
76.98 kDa
pI
8.89
function
formation of a rod-shaped peptidoglycan cell wall
product
penicillin-binding protein H
essential
no
synonyms
pbpH, ykuA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,467,805 1,469,862
Phenotypes of a mutant
a [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA] [gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH] double mutant is not viable [pubmed|36993441]
The protein
Catalyzed reaction/ biological activity
required for [wiki|cell wall synthesis] during cell elongation [Pubmed|12896990]
driver of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] dynamics [Pubmed|21636744,21636745]
Protein family
[wiki|transpeptidase family] (according to UniProt)
Structure
[PDB|2WAF] (Pbp2B from Streptococcus pneumoniae, 31% identity) [pubmed|19233207]
[AF|Q796K8]
Paralogous protein(s)
[protein|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]
[wiki|Localization]
during vegetative growth: evenly distributed along the membrane [pubmed|14731276]
localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]
Additional information
loss of activity at alkaline pH8.5 [pubmed|36993441]
Expression and Regulation
Operons
genes
[gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]
description
[pubmed|22383849]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
Biological materials
Mutant
MGNA-B335 (ykuA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1334 NBRP B. subtilis, Japan]
BKE13980 ([gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGATTGCTCTGTTTC, downstream forward: _UP4_TAAAAAACAGGGTGCACAAC
BKK13980 ([gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGATTGCTCTGTTTC, downstream forward: _UP4_TAAAAAACAGGGTGCACAAC
GFP fusion
3140 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::pSG5058 (cat Pxyl-gfpa-[gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]1-827) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
References
Reviews
Original Publications
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Time of last update: 2024-12-30 15:50:19
Author of last update: Jstuelk