tagE
168
UDP-glucose:polyglycerol phosphate glucosyltransferase
locus
BSU_35730
Molecular weight
78.12 kDa
pI
6.03
function
biosynthesis of teichoic acid
product
UDP-glucose:polyglycerol phosphate glucosyltransferase
essential
no
ec
2.4.1.52
synonyms
tagE, rodD, gtaA, gtaD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0438 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,678,399 3,680,420
Phenotypes of a mutant
inactivation of ''tagE'' is advantageous in environments with fluctuating glucose availability [Pubmed|23924781]
resistant to phage SPO1 [pubmed|30811056]
additional information
the gene readily acquires mutations during cultivation with Arabidopsis thaliana roots [pubmed|37768063]
The protein
Catalyzed reaction/ biological activity
transfer of glucose from UDP-glucose to glycerol phosphate in polymeric form [Pubmed|21558268]
4-O-{[(2R)-1-glycerylphospho](n)-(2R)-1-glycerylphospho}-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl undecaprenyl diphosphate + n UDP-α-D-glucose --> 4-O-{[(2R)-2-α-D-glucosyl-1-glycerylphospho](n)-(2R)-1-glycerylphospho}-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl undecaprenyl diphosphate + n H+ + n UDP (according to UniProt)
Protein family
[wiki|glycosyltransferase 1 family] (according to UniProt)
[wiki|Glycosyltransferase 4 subfamily] (according to UniProt)
Structure
[PDB|4X7R] (from Staphylococcus aureus, 26% identity) [pubmed|25624472]
[AF|P13484]
Modification
phosphorylation on Ser-2 [Pubmed|17218307]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|tagD]-[gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]-[gene|E730E41546298960A4B907FCB7F36C495AA9602B|tagF]-[gene|6795F1703416DCD04E51C177F1C9FE45E73ABAEF|tagG]-[gene|4D3C316965834130BB378CBD1265CFA13F086C7A|tagH]
description
[Pubmed|22383849]
regulation
repressed by phosphate ([protein|search|PhoP]) [Pubmed|9457886]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: repression, [Pubmed|9457886], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [Pubmed|12950927], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7581998], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
1A981 ( ''tagE''::''erm''), [Pubmed|18487323], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A981&Search=1A981 BGSC]
BKE35730 ([gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTTACTCCCTTTCG, downstream forward: _UP4_GTCACGGAAATAAAAGAGAG
BKK35730 ([gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTTACTCCCTTTCG, downstream forward: _UP4_GTCACGGAAATAAAAGAGAG
References
Reviews
Original Publications
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Time of last update: 2024-12-17 13:10:50
Author of last update: Jstuelk