spoIVFB
168
intramembrane metalloprotease (site 2 protease), processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]
locus
BSU_27970
Molecular weight
33.49 kDa
pI
8.48
function
processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]
product
intramembrane metalloprotease
essential
no
synonyms
spoIVFB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
2,855,973 2,856,839
The protein
Catalyzed reaction/ biological activity
processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK] [Pubmed|19805276]
Protein family
[wiki|peptidase M50B family] (according to UniProt)
[wiki|Domains]
C-terminal [wiki|CBS domain], this domain binds ATP [Pubmed|19805276]
[wiki|Cofactors]
Zn2+ [pubmed|35007155]
Structure
[PDB|8VJL] ([protein|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]-Pro-[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]) [pubmed|39419996]
[AF|P26937]
Effectors of protein activity
ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level [Pubmed|19805276]
[protein|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB] is kept inactive by the interaction with [protein|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA] and [protein|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|bofA] [pubmed|35471152,15087499]
[wiki|Localization]
mother cell integral membrane protein [Pubmed|24243021,11959848]
Expression and Regulation
Operons
genes
[gene|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA]-[gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]
description
[Pubmed|1942049]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|15699190,1942049,15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,1942049], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
additional information
the domains that inhibits [protein|search|SpoIVFB] is cleaved off by [protein|search|CtpB] (second cleavage) [PubMed|24243021]
genes
[gene|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA]-[gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]-[gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]-[gene|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp]-[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]
description
[Pubmed|22383849]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|15699190,1942049,15383836]
additional information
the domains that inhibits [protein|search|SpoIVFB] is cleaved off by [protein|search|CtpB] (second cleavage) [PubMed|24243021]
Biological materials
Mutant
BKE27970 ([gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC
BKK27970 ([gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC
References
Reviews
Original Publications
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Time of last update: 2024-12-20 16:50:31
Author of last update: Jstuelk