capB
168
part of the [protein|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[protein|D66994D077D1382B88FF65075E44C2A31089548F|capC] glutamic acid ligase, capsular polyglutamate biosynthesis
locus
BSU_35900
Molecular weight
43.79 kDa
pI
5.07
function
capsule synthesis
product
poly-gamma-glutamate synthetase
essential
no
synonyms
capB, ywsC, pgsB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0771 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,699,446 3,700,627
Phenotypes of a mutant
no synthesis of the poly-gamma-glutamate capsule [Pubmed|11751809]
The protein
Catalyzed reaction/ biological activity
synthesis of poly-gamma-glutamate from L-Glu [Pubmed|11751809]
Structure
[AF|P96736]
Expression and Regulation
Operons
genes
[gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[gene|D66994D077D1382B88FF65075E44C2A31089548F|capC]-[gene|A696434A086A428D411CAF45B37CD8F82AC2503F|capA]-[gene|6B61DCE8D27776EBF942621D3AE90F410FD13F37|capE]
description
[Pubmed|11751809]
regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|19734658], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|19420703], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression of the operon is increased in '[protein|search|motA]' or '[protein|search|motB]' mutants due to increased [protein|search|DegU] phosphorylation [PubMed|24296669]
Biological materials
Mutant
MGNA-B262 (ywsC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1261 NBRP B. subtilis, Japan]
BKE35900 ([gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTCGACATCTCC, downstream forward: _UP4_GTAAGCTAGGGGGAAATGCA
BKK35900 ([gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTCGACATCTCC, downstream forward: _UP4_GTAAGCTAGGGGGAAATGCA
References
Reviews
Original Publications
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Time of last update: 2024-12-23 21:33:26
Author of last update: Jstuelk