pgdS
168
gamma-DL-glutamyl hydrolase
locus
BSU_35860
Molecular weight
45.09 kDa
pI
8.52
function
polyglutamic acid degradation
product
gamma-DL-glutamyl hydrolase
essential
no
synonyms
pgdS, ywtD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0791 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,696,257 3,697,498
The protein
Protein family
[wiki|Peptidase C40 family] (according to UniProt)
[wiki|Domains]
three [wiki|NlpC/P60 domain]s (only the central domain is active) [pubmed|29458655]
Structure
[PDB|4HPE] (from Clostridium difficile, 39% identity)
[AF|P96740]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|86B1182DA7283078B0D393DEB607664059AB910E|pgdS]
description
[Pubmed|15033535]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|15033535], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Biological materials
Mutant
MGNA-A545 (ywtD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/545 NBRP B. subtilis, Japan]
BKE35860 ([gene|86B1182DA7283078B0D393DEB607664059AB910E|pgdS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATTATCTCCTCCT, downstream forward: _UP4_TAAAAGAAAAAGACTCCAAT
BKK35860 ([gene|86B1182DA7283078B0D393DEB607664059AB910E|pgdS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATTATCTCCTCCT, downstream forward: _UP4_TAAAAGAAAAAGACTCCAAT
lacZ fusion
available in [wiki|Daniel Kearns]'s lab [Pubmed|24296669]
References
Reviews
Original Publications
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Time of last update: 2025-04-09 20:57:51
Author of last update: Jstuelk