yyaN
168
similar to transcriptional regulator ([wiki|MerR family])
locus
BSU_40800
Molecular weight
15.91 kDa
pI
9.31
function
unknown
product
unknown
essential
no
synonyms
yyaN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,189,796 4,190,212
The protein
Protein family
[wiki|MerR family]
[wiki|Domains]
[wiki|HTH merR-type domain] (aa 3-72) (according to UniProt)
Structure
[PDB|3QAO] (from Listeria monocytogenes, 29% identity)
[AF|P37510]
Expression and Regulation
Operons
genes
[gene|852ECFA6F254167090AA393E6E0D269F49A4CC6E|yyaN]-[gene|D9D907A6004AFE7A4D7BB92A8955593F00DC7A2C|yyaM]-[gene|56A5266F8F42E0FCFDE941CB71AD103120CEB5E9|yyaL]
description
[Pubmed|22383849]
additional information
translation is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
MGNA-B860 (yyaN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1859 NBRP B. subtilis, Japan]
BKE40800 ([gene|852ECFA6F254167090AA393E6E0D269F49A4CC6E|yyaN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATCCCTCTCATTTAAT, downstream forward: _UP4_TTGAAAGAGGAAGTGGAAAA
BKK40800 ([gene|852ECFA6F254167090AA393E6E0D269F49A4CC6E|yyaN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATCCCTCTCATTTAAT, downstream forward: _UP4_TTGAAAGAGGAAGTGGAAAA
Expression vectors
pGP3933 (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP3935 (C-terminal Strep-tag, purification from E. coli, in [wiki|pGP574]), available in [wiki|Jörg Stülke]'s lab
Page visits: 2140
Time of last update: 2024-11-20 12:58:03
Author of last update: Dennis.wicke