obg
168
GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit
locus
BSU_27920
Molecular weight
47.53 kDa
pI
4.86
function
ribosome assembly, may be required to stimulate activity
product
GTP-binding protein
essential
Yes
ec
3.6.5.-
synonyms
obg
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0536 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,852,661 2,853,947
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes [metabolite|GTP] and readily exchanges [metabolite|GDP] for [metabolite|GTP] [pubmed|39241998]
binds to the premature 50S ribosomal subunit and facilitates recruitment of rproteins and rRNA processing to form the mature 50S subunit [pubmed|39241998]
binds [metabolite|GDP] [pubmed|39241998]
binds [metabolite|ppGpp] [pubmed|39241998]
binds [metabolite|GTP] + Mg(2+) [pubmed|39241998]
Protein family
TRAFAC class OBG-HflX-like GTPase superfamily (with [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA] and [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], according to UniProt)
[wiki|Domains]
Obg possesses 3 domains: A N-terminal glycine-rich domain (1158), a GTP binding domain (159342) and a C-terminal TGS domain (343428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
Obg domain (aa 1-158) (according to UniProt)
OBG-type G domain (aa 159-329) (according to UniProt)
OCT domain (aa 350-428) (according to UniProt)
[wiki|Cofactors]
GDP, GTP and probably ppGpp
Structure
[PDB|1LNZ] [pubmed|12429099]
[AF|P20964]
Paralogous protein(s)
[protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]
[wiki|Localization]
cytoplasm, throughout the cell [pubmed|36406443]
Additional information
The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in ''B. subtilis''.
Expression and Regulation
Operons
genes
[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2537815], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression is repressed by binding of [wiki|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP835, available in [wiki|Jörg Stülke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP843, available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Bill Haldenwang], San Antonio, USA
[wiki|Naotake Ogasawara], Nara, Japan
References
Reviews
Original Publications
Page visits: 4044
Time of last update: 2024-12-20 15:21:58
Author of last update: Jstuelk