obg

obg
168

GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit

locus
BSU_27920
Molecular weight
47.53 kDa
pI
4.86
Protein length
Gene length
function
ribosome assembly, may be required to stimulate activity
product
GTP-binding protein
essential
Yes
ec
3.6.5.-
synonyms
obg

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0536 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,852,661  2,853,947
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes [metabolite|GTP] and readily exchanges [metabolite|GDP] for [metabolite|GTP] [pubmed|39241998]
binds to the premature 50S ribosomal subunit and facilitates recruitment of rproteins and rRNA processing to form the mature 50S subunit [pubmed|39241998]
binds [metabolite|GDP] [pubmed|39241998]
binds [metabolite|ppGpp] [pubmed|39241998]
binds [metabolite|GTP] + Mg(2+) [pubmed|39241998]
Protein family
TRAFAC class OBG-HflX-like GTPase superfamily (with [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA] and [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], according to UniProt)
[wiki|Domains]
Obg possesses 3 domains: A N-terminal glycine-rich domain (1158), a GTP binding domain (159342) and a C-terminal TGS domain (343428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
Obg domain (aa 1-158) (according to UniProt)
OBG-type G domain (aa 159-329) (according to UniProt)
OCT domain (aa 350-428) (according to UniProt)
[wiki|Cofactors]
GDP, GTP and probably ppGpp
Structure
[PDB|1LNZ] [pubmed|12429099]
[AF|P20964]
Paralogous protein(s)
[protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]
[wiki|Localization]
cytoplasm, throughout the cell [pubmed|36406443]
Additional information
The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in ''B. subtilis''.
Expression and Regulation
Operons
genes
[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2537815], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression is repressed by binding of [wiki|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]
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[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]→[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]

2024-08-07 14:04:45

ghost

88

22e8673d70be078a28f38f2ef0353054aac7a361

EFF1EF2402AB3F0071535ACBC9F9FB0A68987C02

Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP835, available in [wiki|Jörg Stülke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP843, available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Bill Haldenwang], San Antonio, USA
[wiki|Naotake Ogasawara], Nara, Japan
References
Reviews
19575570,21885683
Original Publications
36815589,15325267,18689482,7961487,7961486,15583165,2537815,10781545,10419966,12427945,12429099,2537815,20830302,36406443,39241998

84E7D672DB40B114DC4D96A4D8834958B47401A7

Page visits: 3969

Time of last update: 2024-10-05 17:02:12

Author of last update: Jstuelk