gpsA
168
glycerol-3-phosphate dehydrogenase (NAD)
locus
BSU_22830
Molecular weight
37.28 kDa
pI
6.23
function
biosynthesis of phospholipids
product
glycerol-3-phosphate dehydrogenase (NAD)
essential
no
ec
1.1.1.94
synonyms
gpsA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0240 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,389,151 2,390,188
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
poor growth [pubmed|28189581]
poorly transformable [pubmed|28189581]
defective in biofilm formation [pubmed|31420537]
The protein
Catalyzed reaction/ biological activity
NAD+ + sn-glycerol 3-phosphate --> dihydroxyacetone phosphate + H+ + NADH (according to UniProt)
NADP+ + sn-glycerol 3-phosphate --> dihydroxyacetone phosphate + H+ + NADPH (according to UniProt)
Protein family
NAD-dependent glycerol-3-phosphate dehydrogenase family (single member, according to UniProt)
Structure
[PDB|3K96] (from ''Coxiella burnetii RSA 493'', 37% identity, 59% similarity)
[AF|P46919]
[wiki|Localization]
cytoplasm [Pubmed|15743965]
Expression and Regulation
Operons
genes
[gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]-[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]
description
[pubmed|22383849]
genes
[gene|5DA8B27AC6A0E0B4115512BD948C31964D387A8E|yphA]-[gene|448375DF74EB0482A6CD1ACFE04D117C1319E2B2|seaA]-[gene|910109496E4A41EC892996DEEB81971FCBD9CB05|ypzH]-[gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]-[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]
description
[pubmed|22383849]
additional information
highly expressed at high iron concentrations [pubmed|31420537]
Biological materials
Mutant
BKE22830 ([gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGTTTGATTCACACC, downstream forward: _UP4_TAAAAGTGTCAATCAAATGG
BKK22830 ([gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGTTTGATTCACACC, downstream forward: _UP4_TAAAAGTGTCAATCAAATGG
References
Page visits: 4859
Time of last update: 2024-12-22 07:42:39
Author of last update: Jstuelk