truA
168
pseudouridylate synthase I, universally conserved protein
locus
BSU_01480
Molecular weight
27.90 kDa
pI
6.65
function
tRNA modification
product
pseudouridylate synthase I
essential
no
ec
5.4.99.12
synonyms
truA, ybaH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0101 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
152,937 153,680
The protein
Catalyzed reaction/ biological activity
tRNA uridine38-40 --> tRNA pseudouridine38-40 (according to UniProt)
Protein family
tRNA pseudouridine synthase TruA family (single member, according to UniProt)
Structure
[PDB|1VS3] (from ''Thermus thermophilus'', 42% identity) [Pubmed|17114947]
[AF|P70973]
Additional information
[wiki|universally conserved protein]
Expression and Regulation
Operons
genes
[gene|84334531D66BE7524021C4B40C3FA05AAFE0D7A6|truA]-[gene|B495FFC63413D518138F8C2F3FBC894038C97E0E|rplM]-[gene|C8E45EDBCD0CFF353D1D66E6D77FD81E976BC0BC|rpsI]
description
[pubmed|22383849]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
stringent response: negative regulation, [Pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
Biological materials
Mutant
BKE01480 ([gene|84334531D66BE7524021C4B40C3FA05AAFE0D7A6|truA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAGCACCTCTAAGCCC, downstream forward: _UP4_TAAACCAGGTGTATAATATT
BKK01480 ([gene|84334531D66BE7524021C4B40C3FA05AAFE0D7A6|truA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAGCACCTCTAAGCCC, downstream forward: _UP4_TAAACCAGGTGTATAATATT
References
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Time of last update: 2024-12-20 18:23:48
Author of last update: Melvin.boenninger