argS
168
arginyl-tRNA synthetase, universally conserved protein
locus
BSU_37330
Molecular weight
62.55 kDa
pI
5.11
function
translation
product
arginyl-tRNA synthetase
essential
yes
ec
6.1.1.19
synonyms
argS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0018 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,833,650 3,835,320
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + L-arginine + tRNAArg --> AMP + diphosphate + L-arginyl-tRNAArg (according to UniProt)
Protein family
[wiki|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)
Structure
[PDB|3FNR] (from ''Campylobacter jejuni'', 39% identity, 56% similarity)
[AF|P46906]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
[wiki|universally conserved protein]
Expression and Regulation
Operons
genes
[gene|D71F54220FCC2B687857F53F53584D6CB90650CD|ywiB]-[gene|83721D87607FE6448C2C0D17E401C87C57A05AF8|argS]
description
[Pubmed|22383849]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Expression vectors
pGP2897: expression of Strep-''argS'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-10-03 11:57:27
Author of last update: Jstuelk