yfjP
168
similar to DNA-3-methyladenine glycosidase II
locus
BSU_08010
Molecular weight
33.03 kDa
pI
5.52
function
unknown
product
unknown
essential
no
ec
3.2.2.21
synonyms
yfjP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0122 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
872,425 873,288
The protein
Catalyzed reaction/ biological activity
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to UniProt)
Protein family
alkylbase DNA glycosidase AlkA family (with [protein|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA], according to UniProt)
Structure
[PDB|3OH6] (from E. coli, corresponds to aa 68 ... 283, 27.4% identity) [pubmed|20843803]
[AF|O31544]
Expression and Regulation
Operons
genes
[gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]-[gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C275 (yfjP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2273 NBRP B. subtilis, Japan]
BKE08010 ([gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGATCCACCCTTTTTT, downstream forward: _UP4_TAATTCGTCTGACAATGAAG
BKK08010 ([gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGATCCACCCTTTTTT, downstream forward: _UP4_TAATTCGTCTGACAATGAAG
References
Reviews
Original Publications
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Time of last update: 2025-01-18 20:52:35
Author of last update: Jstuelk