kdgA
168
2-dehydro-3-deoxy-phosphogluconate aldolase
locus
BSU_22100
Molecular weight
20.72 kDa
pI
5.27
function
utilization of galacturonic acid
product
2-dehydro-3-deoxy-phosphogluconate aldolase
essential
no
ec
4.1.3.16
synonyms
kdgA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0800 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,323,009 2,323,599
The protein
Catalyzed reaction/ biological activity
4-hydroxy-2-oxoglutarate --> glyoxylate + pyruvate (according to UniProt)
2-dehydro-3-deoxy-6-phospho-D-gluconate --> D-glyceraldehyde 3-phosphate + pyruvate (according to UniProt)
Protein family
KHG/KDPG aldolase family (single member, according to UniProt)
Structure
[PDB|1VLW] (from Thermotoga maritima, 41% identity)
[AF|P50846]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR]-[gene|8A0BB2BE0AAAA7FF834A4549E8E2AF343FB10571|kdgK]-[gene|826BFCE6ED2113BB0510E798C6CDB05C4665E0E2|kdgA]-[gene|EB6F40F8651654E0AB09447C5AD670C35A59F261|kdgT]
description
[Pubmed|9846747]
regulation
induced by galacturonate ([protein|891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR]) [Pubmed|9846747]
regulatory mechanism
[protein|891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR]: repression, [Pubmed|9846747], in [regulon|protein:891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|9846747], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9846747], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE22100 ([gene|826BFCE6ED2113BB0510E798C6CDB05C4665E0E2|kdgA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTTTCAACGACTTTGGACT, downstream forward: _UP4_TAAGTGGAATTGTTTTTTCA
BKK22100 ([gene|826BFCE6ED2113BB0510E798C6CDB05C4665E0E2|kdgA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTTTCAACGACTTTGGACT, downstream forward: _UP4_TAAGTGGAATTGTTTTTTCA
References
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Time of last update: 2024-12-20 08:32:06
Author of last update: Melvin.boenninger