sasB
168
(p)ppGpp synthetase, contributes to cellular heterogeneity in protein synthesis during nutrient limitation
locus
BSU_11600
Molecular weight
24.56 kDa
pI
6.71
function
[metabolite|ppGpp] synthesis independent from stringent response
product
(p)ppGpp synthetase
essential
no
synonyms
sasB, yjbM, relQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2357 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,237,006 1,237,641
Phenotypes of a mutant
a [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] [gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] [gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB] triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of [gene|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB] or inactivation of [gene|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY] [Pubmed|24163341]
a [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] [gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] [gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB] triple mutant acquires suppressor mutations in [gene|AC67305E4C165CC1D785E49B7CD8335346134376|guaA], [gene|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB], [gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk] or inactivation of [gene|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY] [Pubmed|24682323,24163341]
a [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] [gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] [gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB] triple mutant is unable to grow in the presence of [metabolite|guanosine], this can be suppressed by inactivation of [gene|F76A03A71DADC32C7166E37B994EFED019FDF8A4|pyk], [gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT], or [gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk] [pubmed|39998177]
The protein
Catalyzed reaction/ biological activity
ATP + GTP --> AMP + guanosine 3'-diphosphate 5'-triphosphate (according to UniProt)
Protein family
RelA/SpoT family (with [protein|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] and [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel], according to UniProt)
[wiki|Domains]
single ppGpp synthetase domain [Pubmed|24163341]
Structure
[PDB|5DEC] [Pubmed|26460002]
[AF|O31611]
Effectors of protein activity
allosterically activated by pppGpp [Pubmed|26460002], this is inhibited by the [protein|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] product pGpp [pubmed|35594298]
Paralogous protein(s)
[protein|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA]
Expression and Regulation
Operons
genes
[gene|E5A57660B69F9E9BC52FD9C8A5E3C8786CE96728|yjbL]-[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-[gene|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]-[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]
description
[Pubmed|18067544]
regulation
expressed during exponential growth
Biological materials
Mutant
MGNA-B159 (yjbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1158 NBRP B. subtilis, Japan]
GP2066 ([gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] [gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB] [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel]::mls), available in [wiki|Jörg Stülke]'s lab
BKE11600 ([gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATACATCCCCCAATTC, downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
BKK11600 ([gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATACATCCCCCAATTC, downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
GP3470 (Δ[gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA]::spec Δ[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::phleo), available in [wiki|Jörg Stülke]'s lab
GP3472 (Δ[gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel]::cat Δ[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::phleo), available in [wiki|Jörg Stülke]'s lab
GP3425 (Δ[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::phleo) , available in [wiki|Jörg Stülke]'s lab
GP3467 ([gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-6xHis-cat), available in [wiki|Jörg Stülke]'s lab
Expression vectors
pGP3452 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
GP3693 (based on [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2025-05-31 06:32:09
Author of last update: Jstuelk