srfAA
168
surfactin synthetase / competence
locus
BSU_03480
Molecular weight
401.68 kDa
pI
4.87
function
antibiotic synthesis, biocontrol of fungal growth
product
surfactin synthetase / competence
essential
no
synonyms
srfAA, comL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4908 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
376,968 → 387,731
Phenotypes of a mutant
altered cell death pattern in colonies [Pubmed|23012477]
reduced swarming of srfAA mutant of ''B. subtilis'' NCIB3610 [Pubmed|15066026]
defective in biofilm formation and sporulation [pubmed|34460312]
The protein
Protein family
[wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)
[wiki|Domains]
3 Carrier domains (aa 971-1046, aa 2010-2085, aa 3038-3112) (according to UniProt)
Structure
[PDB|2VSQ] (termination module of [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|srfAC], 35% identity) [pubmed|18583577]
Modification
phosphorylation on Ser-1006 [Pubmed|17218307]
[wiki|Localization]
membrane [Pubmed|18763711]
Additional information
the protein can be degraded by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] and other adaptor-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] complexes [pubmed|39096241]
Expression and Regulation
Operons
genes
[gene|81845F44CE2C601555066E31E87384FF5D7B4139|srfAA]-[gene|3C484FDB3A440269E87D6B6E8500A875AC456666|srfAB]-[gene|4D76F07D6124D56C7CEBBDAB9E84B2718BF1E7FC|comS]-[gene|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|srfAC]-[gene|9D0914FC38FFF4028C2BA5970DC87B3A1027E253|srfAD]
description
[Pubmed|1715856]
regulation
expressed at high cell density ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]) [Pubmed|16091051]
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots [pubmed|34557426]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|25666134], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|8830686], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]: activation, [Pubmed|1715856,16091051], in [regulon|protein:7832489F11F606BCF637EC23BAAB41AD10237EBB|comA regulon]
[protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: activation, [Pubmed|16166527], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: repression, [Pubmed|12642660], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
[protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]: repression, [Pubmed|20817675], in [regulon|protein:5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1715856], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|D9A187961CFB9496A6712E9E48AAD384357A3E1C|hxlR]' and '[protein|81845F44CE2C601555066E31E87384FF5D7B4139|srfAA]' [PubMed|20525796]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
BKE03480 (Δ[gene|81845F44CE2C601555066E31E87384FF5D7B4139|srfAA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGTCATACCTCCCCT, downstream forward: _UP4_GAAATTTTCTAATAGAGGTG
BKK03480 (Δ[gene|81845F44CE2C601555066E31E87384FF5D7B4139|srfAA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGTCATACCTCCCCT, downstream forward: _UP4_GAAATTTTCTAATAGAGGTG
References
Reviews
Original Publications
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Time of last update: 2024-12-30 21:25:09
Author of last update: Jstuelk