bshB2
168
N-acetylglucosamine-malate deacetylase, minor enzyme involved in bacillithiol synthesis, may act as amidase for the processing of BSH conjugates
locus
BSU_19460
Molecular weight
16.54 kDa
pI
4.51
function
biosynthesis of bacillithiol
product
N-acetylglucosamin-malate deacetylase
essential
no
synonyms
bshB2, yojG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2120 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,121,641 2,122,306
The protein
Catalyzed reaction/ biological activity
(S)-malyl N-acetyl-α-D-glucosaminide + H2O --> (S)-malyl α-D-glucosaminide + acetate (according to UniProt)
Protein family
PIGL family (with [protein|60367F0396612C8A6491FD92A73B30BED7215506|bshB1], according to UniProt)
Structure
[AF|O31857]
Paralogous protein(s)
[protein|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]
Expression and Regulation
Operons
genes
[gene|7922A49FBC7CCDC8C585EF6E67D7CC9CC91059F7|yoyC]-[gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]-[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]
description
(major transcript) [Pubmed|23894131,20308541]
regulation
''[protein|search|yoyC]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|FBF1A8C92149A216D8A46F9E02898D7C5C448E2A|yojE]-[gene|7922A49FBC7CCDC8C585EF6E67D7CC9CC91059F7|yoyC]-[gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]-[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]
description
[Pubmed|23894131]
regulation
''[protein|search|yoyC]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B428 (yojG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1427 NBRP B. subtilis, Japan]
''bshB2'' null mutant and ''[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]'' / ''[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]'' double null mutant available in [wiki|John Helmann] lab
CS212 (''[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]''::''aphA3'', available in [wiki|Colin Harwood]'s and [wiki|Jörg Stülke]'s labs) [Pubmed|27197833]
BKE19460 ([gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATTATTATTCACCCTT, downstream forward: _UP4_TAATCAAAAAGGTATAAGGC
BKK19460 ([gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATTATTATTCACCCTT, downstream forward: _UP4_TAATCAAAAAGGTATAAGGC
References
Reviews
Original Publications
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Time of last update: 2024-12-30 14:28:48
Author of last update: Jstuelk