mscL
168
large conductance mechanosensitive channel protein, prevents selective release of cytoplasmic proteins in a hypotonic environment
locus
BSU_36360
Molecular weight
14.14 kDa
pI
4.65
function
resistance to osmotic downshock, glycine betaine export
product
large conductance mechanosensitive channel protein
essential
no
synonyms
mscL, ywpC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1970 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,743,267 3,743,659
Phenotypes of a mutant
sensitive to osmotic downshock (> 0.5 M) during logarithmic growth [Pubmed|19252899]
The protein
Protein family
mscL family (single member, according to UniProt)
Structure
[PDB|3HZQ] (MscL of ''Staphylococcus aureus'') [Pubmed|19701184]
[AF|P94585]
[wiki|Localization]
cell membrane [Pubmed|19252899]
Expression and Regulation
Operons
genes
[gene|BDA23FA513C240B07DC109BB62A7A5EB96B20965|ywpB]-[gene|7F7BC285A1D02B031575BA7E980293CD2962C84A|mscL]
description
[Pubmed|22383849]
regulation
expressed in logarithmic phase [Pubmed|19252899]
Biological materials
Mutant
MGNA-A562 (ywpC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/562 NBRP B. subtilis, Japan]
1A957 ( ''mscL''::''spec''), [Pubmed|18310427], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A957&Search=1A957 BGSC]
BKE36360 ([gene|7F7BC285A1D02B031575BA7E980293CD2962C84A|mscL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAATCACCTGCTTTTC, downstream forward: _UP4_TAAAAAAGATGCCGTTAGAA
BKK36360 ([gene|7F7BC285A1D02B031575BA7E980293CD2962C84A|mscL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAATCACCTGCTTTTC, downstream forward: _UP4_TAAAAAAGATGCCGTTAGAA
References
Reviews
Original Publications
Labs working on this gene/protein
[wiki|Jan Maarten van Dijl], Groningen, Netherlands
[wiki|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
Page visits: 3386
Time of last update: 2024-10-06 10:24:47
Author of last update: Jstuelk