yycR
168
formaldehyde dehydrogenase
locus
BSU_40250
Molecular weight
42.79 kDa
pI
5.79
function
detoxification of formaldehyde
product
formaldehyde dehydrogenase
essential
no
synonyms
yycR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1063 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,135,798 4,137,024
Phenotypes of a mutant
reduced tolerance to formaldehyde [pubmed|39470218]
The protein
Catalyzed reaction/ biological activity
[metabolite|formaldehyde] + H2O + NAD+ --> [metabolite|formate] + 2 H+ + NADH [pubmed|39470218]
Protein family
[wiki|zinc-containing alcohol dehydrogenase family] (according to UniProt)
[wiki|Cofactors]
NAD+ [pubmed|39470218]
Zn(2+) [pubmed|39470218]
Structure
[PDB|4JLW] (from Pseudomonas aeruginosa, 66% identity) [pubmed|23989142]
[AF|Q45604]
Kinetic information
Km: 0.19 ± 0.05 mM [pubmed|39470218]
Vmax: 2.24 ± 0.05 nmol min−1 [pubmed|39470218]
Paralogous protein(s)
[protein|EA4CC0D36C39957DBBF9B1B11F65F33E8CD81FFE|adhB], (33%),alcohol dehydrogenase
Expression and Regulation
Operons
genes
[gene|7F4C5AD47D167683F96CC2841039512E70CC4F1C|yycR]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-B816 (yycR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1815 NBRP B. subtilis, Japan]
BKE40250 ([gene|7F4C5AD47D167683F96CC2841039512E70CC4F1C|yycR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATGTGTTTGGACTCT, downstream forward: _UP4_TAAAAGAAAAAGCCGGACGT
BKK40250 ([gene|7F4C5AD47D167683F96CC2841039512E70CC4F1C|yycR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATGTGTTTGGACTCT, downstream forward: _UP4_TAAAAGAAAAAGCCGGACGT
References
Research papers
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Time of last update: 2025-01-01 14:17:20
Author of last update: Jstuelk