amyX

amyX
168

pullulanase (debranching enzyme)

locus
BSU_29930
Molecular weight
80.92 kDa
pI
5.8
Protein length
Gene length
function
starch degradation
product
pullulanase
essential
no
synonyms
amyX

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1523 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,061,651  3,063,807
The protein
Catalyzed reaction/ biological activity
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen (according to UniProt)
Protein family
[wiki|glycosyl hydrolase 13 family] (according to UniProt)
Structure
[PDB|2E8Z] (complex with alpha cyclodextrin),  [PDB|2E9B] (complex with maltose)
[AF|C0SPA0]
Paralogous protein(s)
[protein|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]
Expression and Regulation
Operons
genes
[gene|29A38D244A07ED0973D0846B652238524F4DBC67|ytlP]-[gene|DB1491F13F8B55A360413E815E47A2B51D24395C|ytlQ]-[gene|ED4C1EA16FF96DA20F12B972CD2458FA3B49DDA5|ytlR]-[gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]
description
[pubmed|22383849]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Open in new tab

[gene|29A38D244A07ED0973D0846B652238524F4DBC67|ytlP]->[gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]

2024-10-08 20:17:02

Bzhu

63

2576b9a8551e42406fc3edaa2ce292ee1e219bc5

1F3E1F8E1B45662409D2F7E8989E008A048927D9

genes
[gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]
description
[Pubmed|9387221]
Open in new tab

[gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]

2024-09-15 03:52:16

ghost

45

f076670076c268ec4fc0ceee36aa9a904e37a442

849F59B4D76022B420E2D600A330FB79B954D940

Biological materials
Mutant
BKE29930 ([gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGGAAGCTCTCTCCTCC,  downstream forward: _UP4_TGACGAAGTTTGTGCTATAG
BKK29930 ([gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGGAAGCTCTCTCCTCC,  downstream forward: _UP4_TGACGAAGTTTGTGCTATAG
References
16582490,9387221,19465663,24793495,31028806,32243934

7D783DD180D950A4B030BF5EA78CCE930A466A07

Page visits: 4053

Time of last update: 2024-10-10 17:21:01

Author of last update: Jstuelk