topB

topB
168

DNA topoisomerase III, decatenase

locus
BSU_04260
Molecular weight
81.29 kDa
pI
9.88
Protein length
Gene length
function
control of DNA recombination, maintenance of genomic stability
product
DNA topoisomerase III
essential
no
synonyms
topB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0551 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
476,558  478,741
Phenotypes of a mutant
in E. coli, deletion results in a mutator phenotype [pubmed|2016043,1321123]
The protein
Catalyzed reaction/ biological activity
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to UniProt)
binds to abasic and RNA base DNA lesions [pubmed|37250769]
Protein family
Type IA topoisomerase family (together with [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB])(according to UniProt)
[wiki|Domains]
N-terminal [wiki|TOPRIM domain] (aa 3-136) [Pubmed|9722641]
Structure
[PDB|1D6M] (from ''E. coli'', 33% identity) [Pubmed|10574789]
[AF|P96583]
Effectors of protein activity
[protein|search|RecQ ]controls [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|Topo III] (de)catenation activity [pubmed|11497238,10360177]
Paralogous protein(s)
[protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
Expression and Regulation
Operons
genes
[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]
description
[pubmed|22383849]
Open in new tab

[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]

2024-10-02 20:53:59

Jstuelk

55

71d2cb3714c21193c7eda59695d987b957c30c12

B27EEDA009DFA54388B6A34AE724CC09AD2847DD

genes
[gene|AC2618C53DA020F2CD5192AEE462D15E8EE0F57F|lrpC]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]
description
[pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|10913073], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|AC2618C53DA020F2CD5192AEE462D15E8EE0F57F|lrpC]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]

2024-09-17 23:52:56

Jstuelk

86

7ea663ea3dd5b88b87bf4ca1c3bba54b149de92b

D61F5F3480351B26116444E791717619ADCA51D9

Biological materials
Mutant
MGNA-C111 (topB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2109 NBRP B. subtilis, Japan]
GP1864 (Δ''topB''::''ermC'') (available in [wiki|Jörg Stülke]'s lab)
GP1896 (Δ''topB''::''cat'') (available in [wiki|Jörg Stülke]'s lab)
JH642 and its derivatives: natural deletion of the 18 kb [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] region encompassing the genes [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]-[gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]-[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]-[gene|6085BDAD40F719555EA4EEA7BBAD74103BD03D0F|mutT]-[gene|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]-[gene|87834D5C47064BEFF26F086A8202C311F28F9D38|ydzK]-[gene|search|mntH ][pubmed|18670626]
BKE04260 (Δ[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT,  downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
BKK04260 (Δ[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT,  downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
References
Reviews
11497238,6305585,16936698,29177730,33480441
Original Publications
17114254,16600296,9722641,10574789,10360177,25232096,2016043,1321123,25232096,23514156,22923519,12167668,30222737,30478267,30957856,37250769

7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF

Page visits: 5017

Time of last update: 2024-10-08 03:46:29

Author of last update: Jstuelk