topB
168
DNA topoisomerase III, decatenase
locus
BSU_04260
Molecular weight
81.29 kDa
pI
9.88
function
control of DNA recombination, maintenance of genomic stability
product
DNA topoisomerase III
essential
no
synonyms
topB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0551 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
476,558 478,741
Phenotypes of a mutant
in E. coli, deletion results in a mutator phenotype [pubmed|2016043,1321123]
The protein
Catalyzed reaction/ biological activity
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to UniProt)
binds to abasic and RNA base DNA lesions [pubmed|37250769]
Protein family
Type IA topoisomerase family (together with [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB])(according to UniProt)
[wiki|Domains]
N-terminal [wiki|TOPRIM domain] (aa 3-136) [Pubmed|9722641]
Structure
[PDB|1D6M] (from ''E. coli'', 33% identity) [Pubmed|10574789]
[AF|P96583]
Effectors of protein activity
[protein|search|RecQ ]controls [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|Topo III] (de)catenation activity [pubmed|11497238,10360177]
Paralogous protein(s)
[protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
Expression and Regulation
Operons
genes
[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]
description
[pubmed|22383849]
genes
[gene|AC2618C53DA020F2CD5192AEE462D15E8EE0F57F|lrpC]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]
description
[pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|10913073], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-C111 (topB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2109 NBRP B. subtilis, Japan]
GP1864 (Δ''topB''::''ermC'') (available in [wiki|Jörg Stülke]'s lab)
GP1896 (Δ''topB''::''cat'') (available in [wiki|Jörg Stülke]'s lab)
JH642 and its derivatives: natural deletion of the 18 kb [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] region encompassing the genes [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]-[gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]-[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]-[gene|6085BDAD40F719555EA4EEA7BBAD74103BD03D0F|mutT]-[gene|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]-[gene|87834D5C47064BEFF26F086A8202C311F28F9D38|ydzK]-[gene|search|mntH ][pubmed|18670626]
BKE04260 (Δ[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
BKK04260 (Δ[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
References
Reviews
Original Publications
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Time of last update: 2024-10-08 03:46:29
Author of last update: Jstuelk