pncB
168
nicotinamide phosphoribosyltransferase, nicotinate phosphoribosyltransferase
locus
BSU_31750
Molecular weight
56.01 kDa
pI
5.09
function
NAD salvage and biosynthesis
product
nicotinamide phosphoribosyltransferase
essential
yes
ec
2.4.2.11
synonyms
pncB, yueK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1488 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,259,403 3,260,875
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
5-phospho-α-D-ribose 1-diphosphate + ATP + H2O + nicotinamide --> nicotinamide mononucleotide + ADP + diphosphate + phosphate [https://www.sciencedirect.com/science/article/pii/S1359511324000539#ab0020 paper]
5-phospho-α-D-ribose 1-diphosphate + ATP + H2O + nicotinate --> ADP + diphosphate + nicotinate β-D-ribonucleotide + phosphate (according to UniProt)
Protein family
NAPRTase family (single member, according to UniProt)
Structure
[PDB|2F7F] (from ''Enterococcus faecalis'', 61% identity)
[AF|O32090]
Expression and Regulation
Operons
genes
[gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]-[gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]
description
[Pubmed|22383849]
genes
[gene|17F2619E74A1604E86ED94E1BEE683D49E437A6C|yueH]-[gene|3918D82756A64E86E96A21ACEB34B0CF992D662D|yueI]-[gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]-[gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B562 (yueK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1561 NBRP B. subtilis, Japan]
References
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Time of last update: 2024-12-29 05:40:40
Author of last update: Jstuelk