ymcB
168
tRNA methylthiotransferase
locus
BSU_17010
Molecular weight
58.00 kDa
pI
6.87
function
tRNA maturation
product
tRNA methylthiotransferase
essential
no
synonyms
miaB, ymcB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0621 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,772,843 1,774,372
The protein
Catalyzed reaction/ biological activity
modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA [Pubmed|20472640]
[sulfur carrier]-SH + AH2 + N6-dimethylallyladenosine37 in tRNA + 2 S-adenosyl-L-methionine --> 2-methylsulfanyl-N6-dimethylallyladenosine37 in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H+ + L-methionine + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
methylthiotransferase family (with [protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV], according to UniProt)
[wiki|Domains]
MTTase N-terminal domain (aa 66-184) (according to UniProt)
[wiki|TRAM domain] (aa 440-503) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|4JC0] (from Thermotoga maritima, 33% for aa 70 ... 504) [pubmed|23542644]
[AF|O31778]
Paralogous protein(s)
[protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]-[gene|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]
description
[Pubmed|22383849]
Biological materials
Mutant
GP1109 (spc), available in [wiki|Jörg Stülke]'s lab
BKE17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT, downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA
BKK17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT, downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA
References
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Time of last update: 2024-12-06 04:30:17
Author of last update: Melvin.boenninger