dacA
168
Class C penicillin-binding protein 5, major D-alanyl-D-alanine carboxypeptidase
locus
BSU_00100
Molecular weight
48.47 kDa
pI
5.67
function
carboxypeptidase
product
penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
essential
no
synonyms
dacA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1686 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
17,534 18,865
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
the mutant cells are thicker and shorter than wild type cells [pubmed|34846166]
sensitivity to most β-lactams, and strong resistance to vancomycin [pubmed|35758683]
defective in swarming motility (reduced rate of colony expansion) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to UniProt)
Protein family
[wiki|Peptidase S11 family] (according to UniProt)
Structure
[PDB|1XP4] (from Streptococcus pneumoniae, 37% identity) [pubmed|15596446]
[AF|P08750]
Paralogous protein(s)
[protein|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]
[wiki|Localization]
secreted (according to Swiss-Prot)
membrane associated [Pubmed|18763711]
during vegetative growth: septal, distinct spots at periphery [pubmed|14731276]
Expression and Regulation
Operons
genes
[gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]-[gene|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS]-[gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT]-[gene|82B92D2FB8DB88689D3A8B77FAD2F63FCCA04C7E|serS]
description
[Pubmed|22383849]
regulation
the leader mRNA is processed upstream of [gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
Biological materials
Mutant
1A742 ( ''dacA''::''cat''), [Pubmed|3087956], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A742&Search=1A742 BGSC]
1A743 ( ''dacA''::''cat''), [Pubmed|3087956], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A743&Search=1A743 BGSC]
BKE00100 ([gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCGTACGACCTCCGTAT, downstream forward: _UP4_TAATCAATTGAAAGAGCTCT
BKK00100 ([gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCGTACGACCTCCGTAT, downstream forward: _UP4_TAATCAATTGAAAGAGCTCT
GFP fusion
2085 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]::pSG1493 (cat Pxyl-gfpa-[gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]1-423) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
References
Reviews
Original Publications
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Time of last update: 2024-12-19 07:42:49
Author of last update: Jstuelk