lcfA
168
long chain acyl-CoA synthetase, involved in surfactin production
locus
BSU_28560
Molecular weight
62.52 kDa
pI
6.12
function
fatty acid degradation
product
long chain acyl-CoA synthetase
essential
no
ec
6.2.1.3
synonyms
lcfA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,918,646 2,920,328
The protein
Catalyzed reaction/ biological activity
long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)
activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]
Protein family
[wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)
Structure
[PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 28% identity) [pubmed|22560731]
[AF|P94547]
Paralogous protein(s)
[protein|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB], [protein|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT], [protein|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]-[gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]-[gene|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|fadB]-[gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]-[gene|ACA8FF923D72CC99F447E47F6A1381FEAD9DAB09|etfA]
description
[Pubmed|17189250]
regulation
induced by long chain acyl-CoA (C14 ... C20) ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]) [Pubmed|17189250]
regulatory mechanism
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]: repression, [Pubmed|17189250], in [regulon|protein:8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|21398533], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21398533], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
available in [wiki|Mohamed Marahiel]'s lab [Pubmed|20797616]
1A852 ( ''lcfA''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]
BKE28560 ([gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA, downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
BKK28560 ([gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA, downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
References
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Time of last update: 2024-12-26 11:45:05
Author of last update: Melvin.boenninger