ugtP

168

the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]

increased levels of cell wall precursors [pubmed|33087775]

cells are bent and distended due to the lack of glucolipids [Pubmed|22362028,27684739]

a [gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP] [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] double mutant has a severe [wiki|cell shape] defect, thi can be suppressed by mutations resulting in reduced expression or activity of [protein|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PbpA] [pubmed|33087775]

increased expression of the [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM], [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV], and [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX] regulons [Pubmed|22362028]

altered [wiki|localization] of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] (irregular clusters instead of helical dots)  [Pubmed|22362028]

the inactivation of ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' suppresses the poor and filamentous growth of the ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]'' double mutant [Pubmed|24097947]

increased conjugation of ICEBs1 [Pubmed|25069588,26833415]

1,2-diacyl-3-O-(β-D-glucopyranosyl)-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + H⁺ + UDP (according to UniProt)

1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-Glc-(1→6)-β-D-Glc-(1→6)-β-D-Glc)-sn-glycerol + H⁺ + UDP (according to UniProt)

1,2-diacyl-sn-glycerol + UDP-α-D-glucose --> 1,2-diacyl-3-O-(β-D-glucopyranosyl)-sn-glycerol + H⁺ + UDP (according to UniProt)

the interaction with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] results in inhibition of [wiki|cell division] and an increase of cell size [Pubmed|22931116]

glycosyltransferase 28 family (with [protein|25CECB2D3C7925408DB96A156745BAD6A6FAB0AC|ykoN] and [protein|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG], according to UniProt)

[PDB|4X1T] (galactolipid synthase from Arabidopsis thaliana, 25% identity) [pubmed|26935252]

[AF|P54166]

oligomerization of [protein|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP] is inhibited by UDP-Glc and by interaction with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ]  [Pubmed|22931116]

the amounts of [protein|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP] are reduced about threefold during growth with poor carbon sources due to [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-dependent degradation (this requires most likely [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX] as the chaperone, but [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] and [protein|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE] are also effective) [pubmed|29625553]

membrane-bound protein, self-assembles into tightly wound spirals in vitro [Pubmed|17662947]

under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] [Pubmed|22931116]

under nutrient poor conditions: forms punctate foci (oligomers), no interaction with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] [Pubmed|22931116]

the amounts of [protein|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP] are reduced about threefold during growth with poor carbon sources due to [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-dependent degradation [pubmed|29625553]

MGNA-A886 (ypfP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/886 NBRP B. subtilis, Japan]

GP1369 (''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]''::''spc''), available in [wiki|Jörg Stülke]'s lab

BKE21920 ([gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTAAATTCACCTCAATG,  downstream forward: _UP4_TAATGGCGTACTTGAGAGCA

BKK21920 ([gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTAAATTCACCTCAATG,  downstream forward: _UP4_TAATGGCGTACTTGAGAGCA

pGP2571, for expression in ''B. subtilis'' (based on [wiki|pBQ200], available in [wiki|Jörg Stülke]'s lab

pGP2600, for expression/ purification from ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab