nrgA

nrgA
168

ammonium transporter, required at low ammonium concentration

locus
BSU_36510
Molecular weight
42.57 kDa
pI
5.73
Protein length
Gene length
function
ammonium uptake
product
ammonium transporter
essential
no
synonyms
nrgA, amtB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0004 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,756,790  3,758,004
The protein
Protein family
ammonia transporter channel (TC 1.A.11.2) family (single member, according to UniProt)
Structure
[PDB|4NH2] (from ''E. coli'') [Pubmed|17220269]
[AF|Q07429]
[wiki|Localization]
cell membrane (according to UniProt),  membrane
Expression and Regulation
Operons
genes
[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]
description
[Pubmed|8282685]
regulation
induced by ammonium limitation
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|8799114], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8282685], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]→[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]

2024-10-13 18:13:40

ghost

82

f7f99c18cba030e60508f26bef1662bcb7a80522

89C9A9EC5C318E8075D00EF7FA51F420FFDBD2A5

Biological materials
Mutant
GP4167 (Δ[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::''spec''), available in  [wiki|Jörg Stülke]'s lab
GP739 (cat), GP255 (Δ[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB], cat), both available in [wiki|Jörg Stülke]'s lab [Pubmed|14600241]
1A920 ( ''nrgA''::''erm''), [Pubmed|8282685], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A920&Search=1A920 BGSC]
BKE36510 ([gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCATTCCTCCGTAT,  downstream forward: _UP4_GATTCTATGTGAGGAGTGAC
BKK36510 ([gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCATTCCTCCGTAT,  downstream forward: _UP4_GATTCTATGTGAGGAGTGAC
lacZ fusion
pGP168 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab [Pubmed|14600241]
labs
[wiki|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
References
Reviews
10637624,17368911,37450375,38131184
Original Publications
12823818,17001076,8799114,14600241,8282685,1670935,17220269,25229891,25755103,33559288

796BD46066B1E6147048664FA78679B42FA9D628

Page visits: 5710

Time of last update: 2024-10-14 21:32:10

Author of last update: Jstuelk