nrgA
168
ammonium transporter, required at low ammonium concentration
locus
BSU_36510
Molecular weight
42.57 kDa
pI
5.73
function
ammonium uptake
product
ammonium transporter
essential
no
synonyms
nrgA, amtB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0004 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,756,790 3,758,004
The protein
Protein family
ammonia transporter channel (TC 1.A.11.2) family (single member, according to UniProt)
Structure
[PDB|4NH2] (from ''E. coli'') [Pubmed|17220269]
[AF|Q07429]
[wiki|Localization]
cell membrane (according to UniProt), membrane
Expression and Regulation
Operons
genes
[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]
description
[Pubmed|8282685]
regulation
induced by ammonium limitation
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|8799114], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8282685], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP4167 (Δ[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::''spec''), available in [wiki|Jörg Stülke]'s lab
GP739 (cat), GP255 (Δ[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB], cat), both available in [wiki|Jörg Stülke]'s lab [Pubmed|14600241]
1A920 ( ''nrgA''::''erm''), [Pubmed|8282685], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A920&Search=1A920 BGSC]
BKE36510 ([gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCATTCCTCCGTAT, downstream forward: _UP4_GATTCTATGTGAGGAGTGAC
BKK36510 ([gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCATTCCTCCGTAT, downstream forward: _UP4_GATTCTATGTGAGGAGTGAC
lacZ fusion
pGP168 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab [Pubmed|14600241]
labs
[wiki|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-22 10:07:06
Author of last update: Jstuelk