htrB
168
membrane-anchored protein quality control protease, serine protease, response to secretion and heat stresses
locus
BSU_33000
Molecular weight
48.55 kDa
pI
4.63
function
protein quality control
product
serine protease
essential
no
ec
3.4.21.107
synonyms
htrB, yvtA, yvtB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0265 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,384,070 3,385,446
The protein
Catalyzed reaction/ biological activity
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val (according to UniProt)
Protein family
Peptidase S1C family (with [protein|76FA52D5A5F9FCA14BA9B45872FE3D99D89B211B|htrA] and [protein|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC], according to UniProt)
[wiki|Domains]
cytoplasmic domain (aa 1 - 71) (according to UniProt)
transmembrane helix (aa 72 - 92) (according to UniProt)
extracellular domain (aa 93 - 458) (according to UniProt)
[wiki|PDZ domain] (aa 350- 449) (according to UniProt)
Structure
[PDB|3QO6] (from ''Arabidopsis thaliana'', 38% identity) [Pubmed|21532594]
[AF|Q9R9I1]
[wiki|Localization]
cell membrane (according to UniProt)
randomly distributed in foci throughout the cell surface [Pubmed|22307758]
Expression and Regulation
Operons
genes
[gene|796216BE9CD6DADFEADC29497253C81BF496A2ED|htrB]
description
[Pubmed|22383849]
regulation
expressed under conditions of secretion stress ([protein|A40CD2C23A19860342F440284302EFFDAC09E88A|cssR]) [Pubmed|12270824]
regulatory mechanism
[protein|A40CD2C23A19860342F440284302EFFDAC09E88A|cssR]: activation, [Pubmed|12270824], in [regulon|protein:A40CD2C23A19860342F440284302EFFDAC09E88A|cssR regulon]
additional information
[protein|796216BE9CD6DADFEADC29497253C81BF496A2ED|htrB] is subject to degradation by [protein|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|wprA] and other extracellular proteases [PubMed|24362423]
Biological materials
Mutant
MGNA-B207 (yvtA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1206 NBRP B. subtilis, Japan]
BKE33000 ([gene|796216BE9CD6DADFEADC29497253C81BF496A2ED|htrB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTACACTCCTTTA, downstream forward: _UP4_TAAGAAAAAACAAAAAGCTG
BKK33000 ([gene|796216BE9CD6DADFEADC29497253C81BF496A2ED|htrB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTACACTCCTTTA, downstream forward: _UP4_TAAGAAAAAACAAAAAGCTG
References
Reviews
Original Publications
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Time of last update: 2024-12-20 07:21:51
Author of last update: Jstuelk