dltA
168
D-alanyl-D-alanine carrier protein ligase, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides and against bacteriophage infection
locus
BSU_38500
Molecular weight
55.64 kDa
pI
4.93
function
biosynthesis of teichoic acid
product
D-alanyl-D-alanine carrier protein ligase
essential
no
ec
6.1.1.13
synonyms
dltA, ipa-5r, dae
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1020 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,952,275 3,953,786
Phenotypes of a mutant
increased sensitivity to lysozyme [Pubmed|21856855]
more sensitive to nisin [Pubmed|23980836]
The protein
Catalyzed reaction/ biological activity
ATP + D-alanine + holo-[protein|862D977CF1E185597329A1EF0915485F72482A03|dltC] --> AMP + D-alanyl-[protein|862D977CF1E185597329A1EF0915485F72482A03|dltC] + diphosphate (according to UniProt) [pubmed|37308592]
Protein family
[wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)
Structure
[PDB|3E7X]
[AF|P39581]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|dltX]-[gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]-[gene|1B9BAEBE029BE9D4AD7E49C38B1E6DB5E8F4D4D3|dltB]-[gene|862D977CF1E185597329A1EF0915485F72482A03|dltC]-[gene|D178EC8A1FF22C99C437E01FC4843C5176564992|dltD]-[gene|2FB3F09DA60A40212FE20217A64DAE714AB807CF|dltE]
description
[Pubmed|29794222]
regulation
expression is reduced in a [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV] mutant [Pubmed|21926231]
the mRNA is processed between [gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|dltX] and [gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]: activation, [Pubmed|16306698], in [regulon|protein:7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb regulon]
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|7797557], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|7797557], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|14762009], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
[protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]: sigma factor, [Pubmed|21926231], in [regulon|protein:D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV regulon]
Biological materials
Mutant
BKE38500 ([gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAGTTATTCTCTCTC, downstream forward: _UP4_CGCATTGGCGAAGAGGTTCT
BKK38500 ([gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAGTTATTCTCTCTC, downstream forward: _UP4_CGCATTGGCGAAGAGGTTCT
labs
[wiki|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
References
Reviews
Original Publications
Page visits: 7254
Time of last update: 2024-12-21 22:38:58
Author of last update: Jstuelk