comEA
168
membrane DNA receptor, plays an important role in the transfer of transforming DNA into the DNA channel and in controlling the rate of DNA uptake
locus
BSU_25590
Molecular weight
21.62 kDa
pI
4.82
function
genetic competence
product
membrane DNA receptor
essential
no
ec
null
synonyms
comEA, comD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1555 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,640,513 2,641,130
Phenotypes of a mutant
strongly reduced transformation rate [Pubmed|22398145]
The protein
Catalyzed reaction/ biological activity
stabilizes the attachment of transforming DNA at localized regions in the periplasm and then mediates uptake, probably by a Brownian ratchet mechanism [pubmed|34126763]
[wiki|Domains]
TM domain: aa 10 - 30 [pubmed|36513643]
extracellular DNA-binding domain: 2 HhH domains (aa 142-171,aa 172-302) (according to UniProt)
Structure
[PDB|8DFK] [pubmed|36513643]
[AF|P39694]
[wiki|Localization]
cell membrane (integral), no distinct position [Pubmed|34928178,24164455,21278288]
localizes along the long axis of competent cells [pubmed|37292776]
Expression and Regulation
Operons
genes
[gene|788725411B2E741103603373E43441FD036E1BA0|comEA]-[gene|954359717E15DFC106A9D8111C6637E46B16F8C9|comEB]-[gene|363C6FE07C5E56FDE6BEC6ACFBA1A5535F0F64AC|comEC]
description
[Pubmed|7968523,19028902]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|8196543], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7968523], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Other regulations
[protein|F21A75744FA0B25D5A251CB57E7A7DC6ABFF1DC7|comN]: unknown, [pubmed|19028902]
Biological materials
Mutant
BKE25590 ([gene|788725411B2E741103603373E43441FD036E1BA0|comEA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGCATGTTCCCCGCT, downstream forward: _UP4_TGATTTGACGGTCATGCATT
BKK25590 ([gene|788725411B2E741103603373E43441FD036E1BA0|comEA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGCATGTTCCCCGCT, downstream forward: _UP4_TGATTTGACGGTCATGCATT
References
Reviews
Original Publications
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Time of last update: 2025-03-17 11:50:19
Author of last update: Jstuelk