gidA
168
glucose-inhibited division protein, part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]), synthesis of mnm5s2U at the Wobble base position (U34) in tRNAs
locus
BSU_41010
Molecular weight
69.58 kDa
pI
6.33
function
tRNA modification
product
part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF])
essential
no
synonyms
gidA, mnmG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0445 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,209,603 4,211,489
Phenotypes of a mutant
inactivation of ''[gene|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA]'' reduces sporulation efficiency to 23.9% that of wild type cells; longer mother cells, with some aberrant septa and forespores [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
(s2U34 in tRNA) + GTP + FAD + CH2-THF + NADH --> 5-carboxymethylaminomethyl-2- thiouridine [pubmed|36181794,38050996]
Protein family
MnmG family (with [protein|B6062575849EA65BB7B44A9723676885799E2759|trmFO], according to UniProt)
[wiki|Cofactors]
FAD (according to UniProt)
Structure
[PDB|3CES] (the protein of ''E. coli'', 52.6% identity) [pubmed|19801413]
[AF|P25812]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]-[gene|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA]-[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]-[gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]
description
[Pubmed|11807071]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [Pubmed|26020636], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
Biological materials
Mutant
MGNA-B876 (gidA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1875 NBRP B. subtilis, Japan]
BKE41010 ([gene|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE41010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCTAGTTCCTCCTT, downstream forward: _UP4_ATAGCCGAGTAGAAAGGATG
BKK41010 ([gene|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK41010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCTAGTTCCTCCTT, downstream forward: _UP4_ATAGCCGAGTAGAAAGGATG
References
Reviews
Original Publications
Page visits: 3714
Time of last update: 2024-12-21 11:04:25
Author of last update: Jstuelk