aroD
168
shikimate dehydrogenase
locus
BSU_25660
Molecular weight
30.49 kDa
pI
6.08
function
biosynthesis of aromatic amino acids
product
shikimate dehydrogenase
essential
no
ec
1.1.1.25
synonyms
aroD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0169 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,644,630 2,645,472
Phenotypes of a mutant
slow growth [pubmed|31358606]
50% reduction in mo5U tRNA levels (because prephenate, the product formed by [protein|5C2F7F9F5FCBFEDF77AC41725EFDA0FAF0FC3BC1|aroH], is used as hydroxyl donor for partial ho5U synthesis) [pubmed|31358606]
The protein
Catalyzed reaction/ biological activity
NADP+ + shikimate --> 3-dehydroshikimate + H+ + NADPH (according UniProt)
Protein family
shikimate dehydrogenase family (single member, according to UniProt)
Structure
[PDB|2HK8] (from ''Aquifex aeolicus'', 42% identity, 61% similarity) [Pubmed|17649975]
[AF|P54374]
Expression and Regulation
Operons
genes
[gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|pgpP]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|yqeL]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]
description
[Pubmed|22383849]
expression
expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
Biological materials
Mutant
BKE25660 ([gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25660 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC
BKK25660 ([gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC
References
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Time of last update: 2025-04-12 23:19:35
Author of last update: Melvin.boenninger