dacB
168
sporulation-specific Class C penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase
locus
BSU_23190
Molecular weight
42.92 kDa
pI
9.73
function
control of peptide cross-linking in spore peptidoglycan
product
penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase
essential
no
synonyms
dacB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1686 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,423,383 2,424,531
Phenotypes of a mutant
inactivation of ''[gene|76825D77907E8CE47B17ED56D7E2A348B1ADA5EC|dacB]'' reduces sporulation efficiency to 19.3% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
modifies degree of cross-linking of glycan strands in peptidoglycan [pubmed|9864321]
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)
Protein family
[wiki|Peptidase S11 family] (according to UniProt)
Structure
[PDB|3MFD] (beta-lactamase superfamily domain)
[AF|P35150]
Paralogous protein(s)
[protein|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|76825D77907E8CE47B17ED56D7E2A348B1ADA5EC|dacB]-[gene|F00DAFFBB136F9331C47D45BF44DC59E3EBB75FA|spmA]-[gene|6AFED2DBF443D3E7FECE211BF8FCF1FD33B7104D|spmB]
description
[Pubmed|7642500]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,7528199]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,7528199], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-A098 (dacB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/98 NBRP B. subtilis, Japan]
1A744 ( ''dacB''::''cat''), [Pubmed|1644769], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A744&Search=1A744 BGSC]
1A745 ( ''dacB''::''cat''), [Pubmed|1644769], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A745&Search=1A745 BGSC]
BKE23190 ([gene|76825D77907E8CE47B17ED56D7E2A348B1ADA5EC|dacB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG, downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC
BKK23190 ([gene|76825D77907E8CE47B17ED56D7E2A348B1ADA5EC|dacB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG, downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC
References
Reviews
Original Publications
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Time of last update: 2024-12-20 15:01:03
Author of last update: Jstuelk