opuCA

opuCA
168

glycine betaine/carnitine/choline/arsenobetaine/arsenocholine [wiki|ABC transporter] (ATP-binding protein)

locus
BSU_33830
Molecular weight
43.09 kDa
pI
6.55
Protein length
Gene length
function
compatible solute transport
product
glycine betaine/carnitine/choline/arsenobetaine/arsenocholine [wiki|ABC transporter] (ATP-binding protein)
essential
no
synonyms
opuCA, yvbE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1125 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,469,860  3,471,002
The protein
Catalyzed reaction/ biological activity
uptake of glycine betaine, arsenocholine and arsenobetaine [pubmed|29159878]
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
[wiki|ABC transporter domain] (aa 2-236) (according to UniProt)
2 [wiki|CBS domain]s (aa 255-314, aa 315-373) (according to UniProt)
Structure
[PDB|2IT1] (from Pyrococcus horikoshii, 35% identity)
[PDB|5KS7] ([wiki|CBS domain]s from L. monocytogenes, with c-di-AMP bound) [pubmed|27378384]
[AF|O34992]
Effectors of protein activity
binds c-di-AMP, which likely inhibits uptake activity [pubmed|31061098]
Paralogous protein(s)
[protein|FB2220730013A98D7C979062A4A451518B92DF09|opuAA], [protein|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]
[wiki|Localization]
associated to the membrane (via [protein|83C45215AC86FACB08CF36EB5F9E6B076D7D0F0F|opuCB]-[protein|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|opuCD]) [Pubmed|10092453,18763711]
Expression and Regulation
Operons
genes
[gene|75DB6231129C28C5D3791BA43A1261CD46A861E8|opuCA]-[gene|83C45215AC86FACB08CF36EB5F9E6B076D7D0F0F|opuCB]-[gene|CBA6108A10AD8B932BC5B19A0E7982D0F56F1E08|opuCC]-[gene|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|opuCD]
description
[Pubmed|10216873]
regulation
induced by salt stress [Pubmed|23960087,10216873]
induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
[protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|opcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|protein:9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|opcR regulon]
[protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]: activation, [Pubmed|23646920], in [regulon|protein:6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA regulon]
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
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[gene|75DB6231129C28C5D3791BA43A1261CD46A861E8|opuCA]→[gene|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|opuCD]

2024-10-04 21:09:48

Jstuelk

79

b9927d3ec864bbf0176dc1d26fc265e785d98904

9D14B27F8C4184140FD87446DE1DB30E21C27343

Biological materials
Mutant
BKE33830 ([gene|75DB6231129C28C5D3791BA43A1261CD46A861E8|opuCA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCCGTGCACCTCCGCAG,  downstream forward: _UP4_TGACCAGGGGAGGAGCTCTT
BKK33830 ([gene|75DB6231129C28C5D3791BA43A1261CD46A861E8|opuCA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCCGTGCACCTCCGCAG,  downstream forward: _UP4_TGACCAGGGGAGGAGCTCTT
Expression vectors
pGP2939 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
27935846,32095817,32603625
Original Publications
10092453,9925583,10216873,18763711,21296969,23960087,23646920,27378384,29159878,31061098,32849357

75DB6231129C28C5D3791BA43A1261CD46A861E8

Page visits: 3541

Time of last update: 2024-10-10 13:21:27

Author of last update: TPed