zagA
168
zinc metallochaperone, ZTP activated GTPase A
locus
BSU_03360
Molecular weight
45.15 kDa
pI
4.4
function
supply of zinc from [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE] to [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE]
product
ZTP activated GTPase A
essential
no
synonyms
zagA, yciC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0523 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
366,063 367,256
The protein
Catalyzed reaction/ biological activity
has GTPase and ZTPase activity [pubmed|31132310]
mobilization of zinc from [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE] (after insertion of [protein|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB] into the [wiki|ribosome]), to provide it to [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE] [pubmed|31132310]
the interaction with FolE facilitates the supply of zinc to [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE] under conditions of zinc limitation [pubmed|31132310]
Protein family
SIMIBI class G3E GTPase family (single member, according to UniProt)
[wiki|Domains]
CobW C-terminal domain (aa 259-374) (according to UniProt)
Structure
[PDB|1NIJ] (YjiA from E. coli, 27% identity) [pubmed|14696199]
[AF|P94400]
Modification
phosphorylated on Arg-58 [Pubmed|22517742]
Effectors of protein activity
ZTP (stimulates interaction with [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE]) [https://www.biorxiv.org/content/biorxiv/early/2019/03/31/594382.full.pdf reference]
Kinetic information
KM for GTP: 40 µM [pubmed|31132310]
KM for ZTP: 36 µM [pubmed|31132310]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|F735802BB483C2C5437240AB2E9FC340EAA5B353|yczL]-[gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]
description
[Pubmed|25649915]
regulation
''[wiki|folE2]'': induced by zinc starvation ([protein|search|Zur]) [Pubmed|12426338]
regulatory mechanism
[protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|protein:A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12426338], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]-[gene|456AF184261976FAA73359E81443F7035F457D07|yciB]-[gene|F735802BB483C2C5437240AB2E9FC340EAA5B353|yczL]-[gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]
description
[Pubmed|9811636]
regulation
[wiki|folE2]: induced by zinc starvation ([protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]) [Pubmed|12426338]
regulatory mechanism
[protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|protein:A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12426338], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B999 (yciC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1998 NBRP B. subtilis, Japan]
BKE03360 ([gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAGTGCCTCCATTAA, downstream forward: _UP4_TAGAAAAAAAGCCGTCCCAT
BKK03360 ([gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAGTGCCTCCATTAA, downstream forward: _UP4_TAGAAAAAAAGCCGTCCCAT
labs
[wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
References
Reviews
Original Publications
Page visits: 3817
Time of last update: 2024-12-22 00:57:17
Author of last update: Melvin.boenninger