levD
168
fructose-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIA of the [category|SW.1.2.2|PTS]
locus
BSU_27070
Molecular weight
16.11 kDa
pI
4.48
function
fructose uptake and phosphorylation
product
fructose-specific [category|SW.1.2.2|PTS], EIIA component
essential
no
ec
2.7.1.69
synonyms
levD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2893 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,762,395 2,762,835
The protein
Protein family
[category|SW.1.2.2|PTS] permease, mannose family [Pubmed|10627040]
[wiki|Domains]
[wiki|PTS EIIA domain] type-4 (aa 1-124) (according to UniProt)
Structure
[PDB|1PDO] (E. coli ManX, 33% identity) [pubmed|8676384]
[AF|P26379]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]-[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]-[gene|8ABA3735067C7AA433E09F219EE7239196377A95|levF]-[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]-[gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]
description
[Pubmed|2117666]
regulation
induced in the presence of fructose ([protein|search|LevR]) [Pubmed|1900939]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|7592486], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR]: activation, [Pubmed|1900939], in [regulon|protein:1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|1924373], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
Biological materials
Mutant
BKE27070 ([gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTATTGCTCCTTTCC, downstream forward: _UP4_GATACAAGAGAGGATGAATT
BKK27070 ([gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTATTGCTCCTTTCC, downstream forward: _UP4_GATACAAGAGAGGATGAATT
References
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Time of last update: 2024-10-07 14:19:32
Author of last update: Melvin.boenninger