braB
168
branched-chain amino acid transporter
locus
BSU_29600
Molecular weight
46.45 kDa
pI
9.65
function
uptake of branched-chain amino acids
product
branched-chain amino acid transporter
essential
no
synonyms
braB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1114 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,028,297 3,029,634
Phenotypes of a mutant
no phenotype for the single mutant, the triple ''[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP] [gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ] [gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]
The protein
Catalyzed reaction/ biological activity
high affinity uptake of isoleucine and valine [Pubmed|25645558]
Protein family
branched chain amino acid transporter family (together with [protein|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]) (according to UniProt)
Structure
[AF|O34545]
Paralogous protein(s)
[protein|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]
description
[Pubmed|9387221]
regulation
induced in the absence of branched-chain amino acids ([wiki|CodY], [wiki|ScoC]) [Pubmed|26473603]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|26473603], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|26473603], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|26473603], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
note that [wiki|CodY] represses the expression of ''[protein|search|scoC]'', therefore ''[protein|search|braB]'' expression is increased at intermediate levels of [wiki|CodY] activity [Pubmed|26473603]
Biological materials
Mutant
GP4152 (Δ[gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
GP4157 (Δ[gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]::''aphA3'', Δ[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::''ermC''), available in [wiki|Jörg Stülke]'s lab
BKE29600 ([gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAAAAAATCCTCCTAA, downstream forward: _UP4_TAATGTCACAGAACGCCTGC
BKK29600 ([gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAAAAAATCCTCCTAA, downstream forward: _UP4_TAATGTCACAGAACGCCTGC
References
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Time of last update: 2024-12-21 01:18:10
Author of last update: Jstuelk