hlpB
168
HNH nuclease-like protein, rescues [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-mediated recombination intermediates
locus
BSU_10660
Molecular weight
11.31 kDa
pI
9.94
function
rescuing of [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-mediated recombination intermediates
product
HNH nuclease-like protein
essential
no
synonyms
hlpB, yisB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
1,148,155 1,148,457
Phenotypes of a mutant
essential, the mutation can be suppressed by second-site mutations in [gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA] or [gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB] [Pubmed|22984257], non-essential according to [Pubmed|28189581]
The protein
Structure
[AF|O06715]
[wiki|Localization]
evenly distributed throughout the cytosol in growing cells [Pubmed|22984257]
forms a single focus on the nucleoid upon induction of DNA damage [Pubmed|22984257]
Expression and Regulation
Operons
genes
[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-[gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|4D89BFFB1073407A7A1762AFD18B49F3414D6787|sbcD]-[gene|ED825D5B3290BA8F9C92477B1FCC433ADB3C8449|sbcC]-[gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]
description
[Pubmed|22383849]
regulation
positive control by [protein|search|ComK] [Pubmed|7746142]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7746142], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP3559 (Δ[gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]::kan), available in [wiki|Jörg Stülke]'s lab
MGNA-A503 (yisB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/503 NBRP B. subtilis, Japan]
BKE10660 ([gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC, downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
BKK10660 ([gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC, downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
References
Page visits: 4827
Time of last update: 2025-04-02 02:31:26
Author of last update: MBenda