nifZ

nifZ
168

cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA

Locus
BSU_29590
Molecular weight
41.33 kDa
Isoelectric point
6.34
Protein length
381 aaSequenceBlast
Gene length
1146 bpSequenceBlast
Function
biosynthesis of 4-thiouridine in tRNA
Product
cysteine desulfurase
Essential
no
Synonyms
nifZ, iscSB, iscS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1104 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,026,957  3,028,102
The protein
Catalyzed reaction/ biological activity
removes sulfuryl group from cysteine and transfers it to ThiI PubMed
[sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)
Protein family
Class-V pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Cofactors
PLP
Structure
1EG5 (PDB) (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) PubMed
O34874 (AlphaFold)
Paralogous protein(s)
SufS, NifS, YrvO, YcbU
Expression and Regulation
Operons
Genes
nifZ-thiI-sspA
Description
PubMed
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nifZ→sspA

2025-04-08 23:11:16

Jstuelk

118

c96a207b604fba3a97ff9f805c130b9d09817187

28FE39136DED7BC0B197A71666940376494D0F7A

Biological materials
Mutant
BKE29590 (nifZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT,  downstream forward: _UP4_TTAAGGGAAATGATGAGGTA
BKK29590 (nifZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT,  downstream forward: _UP4_TTAAGGGAAATGATGAGGTA
References
Reviews
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Original Publications
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741DCFFBB8FF6A4E357EE5110EBE24412DE34244

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Time of last update: 2025-04-12 07:27:23

Author of last update: Jstuelk