nifZ
168
cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA
locus
BSU_29590
Molecular weight
41.33 kDa
pI
6.34
function
biosynthesis of 4-thiouridine in tRNA
product
cysteine desulfurase
essential
no
synonyms
nifZ, iscSB, iscS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1104 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,026,957 3,028,102
The protein
Catalyzed reaction/ biological activity
removes sulfuryl group from cysteine and transfers it to [protein|A64A59C2B15015F13911EC53763D370F780400FD|thiI] [Pubmed|22773787]
[sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)
Protein family
[wiki|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|1EG5] (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) [Pubmed|10715213]
[AF|O34874]
Paralogous protein(s)
[protein|88ACE6B79534338F7C72F107B35A0B2384007088|sufS], [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS], [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU]
Expression and Regulation
Operons
genes
[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE29590 ([gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT, downstream forward: _UP4_TTAAGGGAAATGATGAGGTA
BKK29590 ([gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT, downstream forward: _UP4_TTAAGGGAAATGATGAGGTA
References
Reviews
Original Publications
Page visits: 1996
Time of last update: 2024-12-22 06:41:41
Author of last update: Jstuelk