trmB
168
tRNA (m7G46) methyltransferase
locus
BSU_29900
Molecular weight
24.36 kDa
pI
6.35
function
tRNA modification
product
tRNA (m7G46) methyltransferase
essential
no
ec
2.1.1.33
synonyms
trmB, ytmQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0220 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,059,547 3,060,188
The protein
Catalyzed reaction/ biological activity
guanosine46 in tRNA + S-adenosyl-L-methionine --> N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
[wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)
[wiki|Cofactors]
S-adenosylmethionine [pubmed|16600901]
Structure
[PDB|2FCA] [pubmed|16600901]
[AF|O34522]
Expression and Regulation
Operons
Biological materials
Mutant
GP2649 ([gene|73ED086EED7CECBE2FCADFBC964F1263CF825DB0|trmB]::''cat''), available in [wiki|Jörg Stülke]'s lab
MGNA-A435 (ytmQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/435 NBRP B. subtilis, Japan]
BKE29900 ([gene|73ED086EED7CECBE2FCADFBC964F1263CF825DB0|trmB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGATTGACACCTCTAT, downstream forward: _UP4_TAAAGACAGACTCTGACAGG
BKK29900 ([gene|73ED086EED7CECBE2FCADFBC964F1263CF825DB0|trmB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGATTGACACCTCTAT, downstream forward: _UP4_TAAAGACAGACTCTGACAGG
References
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Time of last update: 2024-10-02 19:51:14
Author of last update: Jstuelk