yhfR
168
phosphatase involved in isopentenol (isoprenoid) biosynthesis
locus
BSU_10340
Molecular weight
21.85 kDa
pI
5.16
function
isoprenoid biosynthesis
product
unknown
essential
no
synonyms
yhfR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0406 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,108,736 1,109,317
Phenotypes of a mutant
no detectable phenotype [Pubmed|10869096]
The protein
Catalyzed reaction/ biological activity
phosphatase involved in isopentenol (isoprenoid) biosynthesis
Protein family
similar to 2,3-diphosphoglycerate-dependent phosphoglycerate mutases [Pubmed|10869096]
Structure
[PDB|1H2F] (enzyme of ''B. stearothermophilus'') [Pubmed|11827481]
[AF|O07617]
Additional information
The gene is annotated in KEGG as an ortholog of phosphoglycerate mutase (PGM) EC 5.4.2.1. In MetaCyc the protein is marked as similar to phosphoglycerate mutase. No EC annotation is available in Swiss-Prot. Pearson et al. ([Pubmed|10869096]) demonstrated that yhfR is non-essential for growth, sporulation, and spore germination. They also purified the gene, expressed it in ''B. subtilis'' but were not able to detect PGM activity in ''B. subtilis''. [Pubmed|19935659]
Expression and Regulation
Operons
genes
[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]
description
[Pubmed|22383849]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]
additional information
weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
genes
[gene|22215066A7BEA4E53189DE6639C19D1AD86DA7F4|panU]-[gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]
description
[Pubmed|22383849]
regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|11717296]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, [Pubmed|11717296], in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]
additional information
weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
genes
[gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]
description
[Pubmed|22383849]
additional information
weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
Biological materials
Mutant
MGNA-A243 (yhfR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/243 NBRP B. subtilis, Japan]
BKE10340 ([gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAATCCCCTTCCTGAC, downstream forward: _UP4_TAAGAAAAAGACAGGCGTTT
BKK10340 ([gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAATCCCCTTCCTGAC, downstream forward: _UP4_TAAGAAAAAGACAGGCGTTT
References
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Time of last update: 2025-04-09 12:58:48
Author of last update: Melvin.boenninger