yflS
168
malate transporter
locus
BSU_07570
Molecular weight
51.27 kDa
pI
9.74
function
malate uptake
product
malate transporter
essential
no
synonyms
yflS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0471 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
829,382 830,818
RNA
additional information
the mRNA is stabilized by basepairing with the RoxS RNA [pubmed|28436820]
non-protected mRNA is subject to degradation by by [wiki|rnjA|RNase J1] [pubmed|28436820]
The protein
Catalyzed reaction/ biological activity
uptake of malate
Protein family
SLC13A/DASS transporter (TC 2.A.47) family (single member, according to UniProt)
Structure
[AF|O34726]
[wiki|Localization]
cell membrane (according to UniProt)
spore membrane [pubmed|30602489]
Expression and Regulation
Operons
genes
[gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]
description
[Pubmed|21815947]
regulation
induced by malate ([protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR])
regulatory mechanism
[protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]: activation, in [regulon|protein:689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR regulon]
additional information
the mRNA is substantially stabilized upon depletion of [wiki|Rny|RNase Y ][PubMed|21815947]
the mRNA is stabilized by basepairing with the [protein|search|RoxS ]RNA [pubmed|28436820]
non-protected mRNA is subject to degradation by by [wiki|rnjA|RNase J1] [pubmed|28436820]
Biological materials
Mutant
MGNA-C249 (yflS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2247 NBRP B. subtilis, Japan]
GP1450 (cat), available in [wiki|Jörg Stülke]'s lab
BKE07570 ([gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG
BKK07570 ([gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG
References
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Time of last update: 2024-10-04 20:11:22
Author of last update: Melvin.boenninger