tagA
168
UDP-N-acetyl-D-mannosamine transferase
locus
BSU_35750
Molecular weight
29.35 kDa
pI
10.13
function
biosynthesis of teichoic acid
product
UDP-N-acetyl-D-mannosamine transferase
essential
yes
ec
2.4.1.187
synonyms
tagA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1922 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,681,370 3,682,140
Phenotypes of a mutant
essential [Pubmed|12682299], reduced growth rate, loss of rod-like morphology [Pubmed|19376878]
inactivation of ''[gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA]'' strongly restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]
knockdown of expression results in loss of [category|SW.5.2.1|ICEBs1] conjugation (both in donor and recipient cells) [pubmed|35490406]
The protein
Catalyzed reaction/ biological activity
N-acetyl-α-D-glucosaminyl-1-diphospho-di-trans,octa-cis-undecaprenol + UDP-N-acetyl-α-D-mannosamine --> H+ + N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl di-trans,octa-cis-undecaprenyl diphosphate + UDP (according to UniProt)
Protein family
glycosyltransferase 26 family (single member, according to UniProt)
Structure
[PDB|5WB4] (from Thermoanaerobacter italicus, corresponds to aa 35 ... 194, 33% identity)
[AF|P27620]
[wiki|Localization]
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA]-[gene|911A4D957A6C0DFC636A152395119DBFC777DDA2|tagB]
description
[Pubmed|7952180]
regulation
repressed when phosphate is available ([protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]) [Pubmed|9457886]
the leader mRNA is processed upstream of [gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: repression, [Pubmed|9457886], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [Pubmed|12950927], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7581998], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
References
Reviews
Original Publications
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Time of last update: 2024-12-20 20:53:57
Author of last update: Jstuelk