folD
168
methylenetetrahydrofolate dehydrogenase (NADP)
locus
BSU_24310
Molecular weight
30.53 kDa
pI
5.6
function
formylation of Met-tRNA(fMet)
product
methylenetetrahydrofolate dehydrogenase (NADP)
essential
no
ec
1.5.1.5
synonyms
folD, yqiA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0190 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,528,404 2,529,255
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
inactivation of ''[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]'' suppresses the synthetic lethality of the ''[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA] [gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' double mutant [Pubmed|27983482]
The protein
Catalyzed reaction/ biological activity
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP+ --> 5,10-methenyltetrahydrofolate + NADPH (according to UniProt)
5,10-methenyltetrahydrofolate + H2O --> (6S)-10-formyltetrahydrofolate + H+ (according to UniProt)
Protein family
tetrahydrofolate dehydrogenase/cyclohydrolase family (single member, according to UniProt)
Structure
[PDB|1B0A] (from ''E. coli'', 52% identity) [Pubmed|10386884]
[AF|P54382]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]-[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]
description
[Pubmed|2536750,11591660]
regulation
repressed in the presence of purine nucleotides ([protein|search|PurR]) [Pubmed|2536750,11591660]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|2536750,11591660], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
Biological materials
Mutant
BKE24310 ([gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCCTCCTATGA, downstream forward: _UP4_TAGATAATTGGAGATATTCA
BKK24310 ([gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCCTCCTATGA, downstream forward: _UP4_TAGATAATTGGAGATATTCA
References
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Time of last update: 2024-12-20 00:09:12
Author of last update: Jstuelk