besA
168
Ferri-bacillibactin esterase
locus
BSU_32010
Molecular weight
35.34 kDa
pI
8.2
function
iron acquisition
product
ferri-bacillibactin esterase
essential
no
synonyms
besA, yuiI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2819 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,292,490 3,293,359
The protein
Catalyzed reaction/ biological activity
catalyses ferri-bacillibactin hydrolysis leading to cytosolic iron release [pubmed|28283524]
Protein family
Esterase D family (single member, according to UniProt)
Structure
[PDB|2QM0] (from B. cereus, 46% identity)
[AF|O32102]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|71F490B2C3199734620724B51F8E3D7977C2D8D8|besA]
description
[Pubmed|12354229]
regulation
induced upon iron starvation ([protein|search|Fur]) [Pubmed|12354229]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
additional information
[protein|search|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
MGNA-A650 (yuiI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/650 NBRP B. subtilis, Japan]
BKE32010 ([gene|71F490B2C3199734620724B51F8E3D7977C2D8D8|besA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTACGACATTCCTC, downstream forward: _UP4_TGAGACATACTCAGCCTTGC
BKK32010 ([gene|71F490B2C3199734620724B51F8E3D7977C2D8D8|besA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTACGACATTCCTC, downstream forward: _UP4_TGAGACATACTCAGCCTTGC
References
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Time of last update: 2024-11-08 06:36:34
Author of last update: Jstuelk