nupO

nupO
168

[wiki|ABC transporter] for guanosine (ATP-binding protein)

locus
BSU_31550
Molecular weight
56.13 kDa
pI
7.5
Protein length
Gene length
function
uptake of guanosine
product
[wiki|ABC transporter] for guanosine
essential
no
synonyms
nupO, yufO

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3845 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,241,085  3,242,617
Phenotypes of a mutant
a ''[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO] [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]
defective in the the ability to support the growth of ''Synechococcus leopoliensis'' CCAP1405/1 on agar media [Pubmed|25875741]
The protein
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
2 [wiki|ABC transporter domain]s (aa 5-240, aa 257-501) (according to UniProt)
Structure
[PDB|5XJY] (human protein, 30% identity) [pubmed|28602350]
[AF|O05253]
Paralogous protein(s)
[protein|AB4A686F62550F90F5BD8D27DAADC9C33F1DA1DB|rbsA]
[wiki|Localization]
associated to the membrane (via [protein|0BB235F189D30C9BE798560C31DA59FF0112CFD9|nupP]-[protein|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]) [Pubmed|10092453]
Expression and Regulation
Operons
genes
[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]-[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]-[gene|0BB235F189D30C9BE798560C31DA59FF0112CFD9|nupP]-[gene|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]
description
[Pubmed|21926227]
regulation
repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455,21699902,21926227]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455,21699902,21926227], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21926227], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]→[gene|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]

2024-10-10 15:51:19

ghost

66

176c32eb4a5e0c99af55c2dcae84f2d09f6ef942

1A88DAA8C1BBE629E7600C276F82763A709A3EA7

Biological materials
Mutant
MGNA-B554 (yufO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1553 NBRP B. subtilis, Japan]
BKE31550 ([gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGATTGACCGCTCCTTT,  downstream forward: _UP4_ACGCAAAAGGAAGCGGGGAA
BKK31550 ([gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGATTGACCGCTCCTTT,  downstream forward: _UP4_ACGCAAAAGGAAGCGGGGAA
References
10092453,21926227,12618455,25875741

71546D1508EF17A208332E162CAE2C299987A569

Page visits: 4270

Time of last update: 2024-10-10 17:12:26

Author of last update: Melvin.boenninger