nupO
168
[wiki|ABC transporter] for [metabolite|guanosine] ([metabolite|ATP]-binding protein)
locus
BSU_31550
Molecular weight
56.13 kDa
pI
7.5
function
uptake of [metabolite|guanosine]
product
[wiki|ABC transporter] for [metabolite|guanosine]
essential
no
synonyms
nupO, yufO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3845 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,241,085 3,242,617
Phenotypes of a mutant
a ''[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO] [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]
defective in the the ability to support the growth of ''Synechococcus leopoliensis'' CCAP1405/1 on agar media [Pubmed|25875741]
The protein
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
2 [wiki|ABC transporter domain]s (aa 5-240, aa 257-501) (according to UniProt)
Structure
[PDB|5XJY] (human protein, 30% identity) [pubmed|28602350]
[AF|O05253]
Paralogous protein(s)
[protein|AB4A686F62550F90F5BD8D27DAADC9C33F1DA1DB|rbsA]
[wiki|Localization]
associated to the membrane (via [protein|0BB235F189D30C9BE798560C31DA59FF0112CFD9|nupP]-[protein|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]) [Pubmed|10092453]
Expression and Regulation
Operons
genes
[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]-[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]-[gene|0BB235F189D30C9BE798560C31DA59FF0112CFD9|nupP]-[gene|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]
description
[Pubmed|21926227]
regulation
repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455,21699902,21926227]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455,21699902,21926227], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21926227], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B554 (yufO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1553 NBRP B. subtilis, Japan]
BKE31550 ([gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTGACCGCTCCTTT, downstream forward: _UP4_ACGCAAAAGGAAGCGGGGAA
BKK31550 ([gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTGACCGCTCCTTT, downstream forward: _UP4_ACGCAAAAGGAAGCGGGGAA
References
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Time of last update: 2024-12-30 19:33:30
Author of last update: Jstuelk