pgcA
168
alpha-phosphoglucomutase, required for UDP-glucose synthesis, inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] ring assembly (indirect effect due to a defect in UDP-glucose synthesis), possesses a secondary phosphoglucosamine mutase activity
locus
BSU_09310
Molecular weight
62.73 kDa
pI
4.91
function
interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate
product
alpha-phosphoglucomutase
essential
no
ec
5.4.2.2
synonyms
pgcA, yhxB, gtaC, gtaE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1109 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,006,774 1,008,519
Phenotypes of a mutant
cells are bent and distended due to the lack of glucolipids [Pubmed|27684739]
the inactivation of ''[gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]'' suppresses the poor and filamentous growth of the ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]'' double mutant [Pubmed|24097947]
inactivation of ''[gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]'' reduces sporulation efficiency to 0.1% that of wild type cells; smaller and wider mother cells with small forespores [Pubmed|26735940]
resistant to phage SPO1 [pubmed|30811056]
a [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA] [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] double mutant is not viable, this can be suppressed by overexpression of [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM] [pubmed|31589605]
a [gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf] [gene|E3DAC5DE86EE0E34C6ECB6DE1E41309742465CF9|pgi] [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA] triple mutant is not viable [pubmed|30782637]
defective in [category|SW.4.1.4|Swarming] motility (prolonged period of immotility prior to [category|SW.4.1.4|Swarming]) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
α-D-glucose 1-phosphate --> α-D-glucose 6-phosphate (according to UniProt)
D-glucosamine 6-phosphate --> α-D-glucosamine 1-phosphate (secondary activity, this activity is increased by a G47S mutation) [pubmed|31589605]
Protein family
phosphohexose mutase family (with [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM], [pubmed|15238632])
Structure
[PDB|2Z0F] (from Thermus thermophilus, 27% identity)
[AF|P18159]
Modification
phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]
Additional information
PgcA inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] ring assembly (indirect effect due to a defect in UDP-glucose synthesis)[Pubmed|17662947]
a PgcA (G47S) variant suppresses the growth defect of a [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] mutant on gluconeogenic carbon sources in the absence of glucose due to the increased phosphoglucosamine mutase activity [pubmed|31589605]
Expression and Regulation
Operons
genes
[gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B476 (yhxB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1475 NBRP B. subtilis, Japan]
GP1743: BSB1 ''pgcA::aphA3'', available in [wiki|Jörg Stülke]'s lab
BKE09310 ([gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGTCCTCCTATGTA, downstream forward: _UP4_TAATTCAAGTATGAGCTGGG
BKK09310 ([gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGTCCTCCTATGTA, downstream forward: _UP4_TAATTCAAGTATGAGCTGGG
References
Reviews
Original Publications
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Time of last update: 2024-12-20 14:10:57
Author of last update: Jstuelk