malK
168
two-component sensor kinase, regulation of malate uptake
locus
BSU_31520
Molecular weight
58.76 kDa
pI
6.95
function
regulation of malate uptake
product
two-component sensor kinase
essential
no
ec
null
synonyms
malK, yufL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3290 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,237,157 3,238,758
Phenotypes of a mutant
unable to use malate as the single carbon source [Pubmed|12949159]
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments
[wiki|PAS domain] (aa 210-281) (according to UniProt)
[wiki|Histidine kinase domain] (aa 335-527) (according to UniProt)
Structure
[AF|O05250]
Modification
autophosphorylation on a His residue
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]-[gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A634 (yufL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/634 NBRP B. subtilis, Japan]
BKE31520 ([gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAACTTCCTTACGT, downstream forward: _UP4_CCGTACGAACCGAAGGAGGA
BKK31520 ([gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAACTTCCTTACGT, downstream forward: _UP4_CCGTACGAACCGAAGGAGGA
labs
[wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
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Time of last update: 2024-10-15 18:10:15
Author of last update: Melvin.boenninger