mbl
168
cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] activity, required for WTA and LTA synthesis/attachment with an impact on growth and cell-shape determination (function not shared with [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB])
locus
BSU_36410
Molecular weight
35.70 kDa
pI
5.67
function
[wiki|cell shape] determination
product
[protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-like protein
essential
yes
synonyms
mbl
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1077 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,747,254 3,748,255
Phenotypes of a mutant
essential [Pubmed|28189581]
non-viable in the absence of low Mg2+, growth can be rescued by the addition of mirubactin C [pubmed|36312962]
readily accumulate [gene|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI] suppressor mutants [Pubmed|19114499]
the growth defect of the mutant can be suppressed by over expression of [protein|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF] [pubmed|36744964]
the growth defect can be rescued by iron-chelating compounds or by reducing the iron content in the medium [pubmed|37433811]
the growth defect can be rescued by overexpression of [gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG] [pubmed|37433811]
the growth defect can be suppressed by inactivation of any of the following genes: [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR], [gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI], [gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS], [gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI], [gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC], [gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB], [gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH], [gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV], [gene|FD30C4E458E63E8D6A932B351D4224F1AACD4B1B|gtcA], [gene|D6B1837075ECBEE1FEAC704DDB6795336092897F|yabM], [gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj], or [gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS] [pubmed|39185233]
The protein
Catalyzed reaction/ biological activity
forms helical filaments in a heterologous system [Pubmed|21091501]
required for [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] activity [Pubmed|23869552]
Protein family
[wiki|FtsA/MreB family] (according to UniProt)
Structure
[PDB|1JCF] ([protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] from ''Thermotoga maritima'', 52% identity) [Pubmed|11544518]
[AF|P39751]
Paralogous protein(s)
[protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB], [protein|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|mreBH]
[wiki|Localization]
during logarithmic growth, [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]
reports on helical structures formed by Mbl [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744]
Mbl patch speed depends on the growth rate, the faster growth, the higher the patch speed [pubmed|28589952]
Expression and Regulation
Operons
genes
[gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]
description
[Pubmed|22383849]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|9023218]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, promoter p1, upstream of [protein|487990143C84D245743C0014E648699627204FF0|usd] [Pubmed|9023218], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p2, upstream of [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
An [wiki|antisense RNA] is predicted for '[protein|search|mbl]' [PubMed|20525796]
genes
[gene|487990143C84D245743C0014E648699627204FF0|usd]-[gene|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]-[gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]
description
[Pubmed|22383849]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|9023218].
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|9023218], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
additional information
An antisense RNA is predicted for '[protein|search|mbl]' [PubMed|20525796]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
BKE36410 ([gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTTATATCCTCCTT, downstream forward: _UP4_TGATTTCACAAACCTCATTC
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in the [wiki|Jeff Errington]'s and [wiki|Peter Graumann]'s labs
labs
[wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
References
Original Publications
Localization
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Time of last update: 2024-12-21 21:51:59
Author of last update: Jstuelk