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ricF
168
subunit of the regulatory iron-sulfur containing [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex, required for handing over of [protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] to [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] to facilitate maturation of polycistronic mRNAs, antagonist of biofilm repression by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR], control of the [wiki|phosphorelay]
Molecular weight
16.77 kDa
function
control of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] activity, see [wiki|Targets of the Y complex]
product
subunit of the regulatory iron-sulfur containing [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex
Genomic Context
This gene is a member of the following
regulons
Gene
Coordinates
1,568,420 1,568,869
Phenotypes of a mutant
strongly reduced [wiki|genetic competence], strongly reduced [wiki|sporulation] [Pubmed|23490197]
defective in [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y]-mediated maturation of polycistronic mRNAs, see [wiki|Targets of the Y complex] [pubmed|29794222]
defective in [category|SW.4.1.4|Swarming] motility (prolonged period of immotility prior to [category|SW.4.1.4|Swarming]) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex acts as specificity factor for [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y]-dependent processing of polycistronic mRNAs, see [wiki|Targets of the Y complex] [pubmed|29794222]
the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex stimulates [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] phosphorylation in the [category|SW.4.2.2|phosphorelay] [pubmed|27501195]
[wiki|Cofactors]
one 4Fe-4S cluster (contributes to the co-ordination of the cluster (with [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA] and [protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT])) [pubmed|31530674]
Structure
[PDB|6PRK] (the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF] complex) [pubmed|31530674]
[AF|O34412]
[wiki|Localization]
cytoplasm [pubmed|35326384,27501195]
Expression and Regulation
Operons
genes
[gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]
description
[Pubmed|22383849]
[gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]
2024-12-14 19:30:24
Jstuelk
76
9b91c064845193c73a6df7b00010fbb52dd009e1
2064818B92DA4E61A39DBD20D7034E1169CB7B4A
genes
[gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[gene|FBD8F0EB241A35747E6398C9AA30D71D6D17CE99|ylbG]-[gene|65FA4B3738C2A5995F019099743C782ADD15C338|ylbH]-[gene|6DAAEF20B68139A732FDCDABD0C420A0C928D373|coaD]
description
[Pubmed|22383849]
[gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]→[gene|6DAAEF20B68139A732FDCDABD0C420A0C928D373|coaD]
2024-12-10 07:41:29
Jstuelk
106
2162b14729468342cf42886fefd68855b3668199
79BDA992AAE4BACCDAC85A63130F2F758FA7CD65
additional information
7,000/ 340 molecules per cell during growth in LB/ minimal medium [pubmed|31530674]
Biological materials
Mutant
BKE14990 ([gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14990 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG
BKK14990 ([gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG
References
Reviews
32061882,32156829,32156813,35638784 Original Publications
10712692,15661000,23490197,15175311,26434553,28295778,27501195,29794222,31530674,35326384,35638827,36815589,37555678
