fliW

fliW
168

checkpoint protein for [gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag] expression, [protein|93F328524989597C7D2329B25E665496C9631E87|csrA] antagonist

locus
BSU_35380
Molecular weight
16.03 kDa
pI
4.34
Protein length
Gene length
function
control of [protein|93F328524989597C7D2329B25E665496C9631E87|csrA] activity
product
flagellar assembly factor, [protein|93F328524989597C7D2329B25E665496C9631E87|csrA] antagonist
essential
no
synonyms
fliW, yviF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1699 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,636,264  3,636,695
The protein
Catalyzed reaction/ biological activity
[protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW] binds [protein|93F328524989597C7D2329B25E665496C9631E87|csrA] when [protein|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag] is secreted after hook completion (and [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW] released from the [protein|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]-[protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW] complex) to allow translation of the'' [gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' mRNA [Pubmed|21895793]
Protein family
fliW family (single member, according to UniProt)
Structure
[PDB|5DMD] (from ''Geobacillus thermodenitrificans'') [Pubmed|27551070]
[PDB|5DMB] ([protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[protein|93F328524989597C7D2329B25E665496C9631E87|csrA] complex) [Pubmed|27551070]
[AF|P96503]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
[protein|93F328524989597C7D2329B25E665496C9631E87|csrA] and [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW] form a conserved module in many bacteria [Pubmed|21895793,27516547]
Expression and Regulation
Operons
genes
[gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|flgN]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]-[gene|E5A59D2AF53FA220BA98D9653589A8F0D24F75B8|yviE]-[gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]
description
[Pubmed|22383849]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|8412657], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|21736639], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|19898538], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8412657], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8045879], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
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[gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]→[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]

2024-09-19 14:48:14

Jstuelk

135

F13A7084BCCB15345BC26C03AF15EE753EFDF196

11180672E46D33F7D747E58441ECB10AEE7F8D8B

Biological materials
Mutant
MGNA-A339 (yviF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/339 NBRP B. subtilis, Japan]
BKE35380 ([gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35380 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTTCACGATCCTTTTC,  downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
BKK35380 ([gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35380 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTTCACGATCCTTTTC,  downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
References
Reviews
22672726,25251856
Original Publications
36815589,16936039,23144244,21895793,26577401,27516547,27551070,31113895,33106347

6A6CBD75DCC33C804F853F47D37773930D398CFE

Page visits: 3747

Time of last update: 2024-10-07 13:23:36

Author of last update: Jstuelk